Abstract:

Objective To study the effect of Actinobacillus actinomycetem（A.actinomycetem） on the secretion and apoptosis rate of polymorphonuclear leukocytes（PMN） in type 2 diabetes patients. Methods Peripheral PMN from healths and type 2 diabetes patients were isolated by Percoll gradient centrifugation. The PMN were stimulated with filtrate of ultrasonic pulverization from  .actinomycetem as the experiment group. As the control group, PMN suspension was incubated with PBS. The release of interleukin-6（IL-6） was measured at 20, 40, 60 min by enzymelinked immune sorbent assay（ELISA） technique. The apoptosis rate of PMN was tested at 6 and 12 hours by flow cytometry. Results Incubated with filtrate of ultrasonic pulverization from A.actinomycetem, the PMN of type 2 diabetes patients released  significantly higher levels of IL-6 compared with the healthy subjects（P＜0.001）. The apoptosis rate of PMN from the healthy subjects was higher than that from type 2 diabetes patients（P<0.001）. Regardless of body condition, interaction with filtrate of ultrasonic pulverization from A.actinomycetem could induce the seretion of IL-6 and reduce the apoptosis rate. Conclusion The PMN of type 2 diabetes patients may possess hyper-reactive inflammatory response trait.

Key words: type 2 diabetes, polymorphonuclear leukocyte, interleukin-6, cell apoptosis