Abstract:

Objective To study the apoptotic effect on the squamous cell carcinoma cell line TCa83 induced by recombined adenovirus vector containing TRAIL gene and CMV promoter. Methods The TCa83 cell line was firstly infected with different titre of AdCMV- EGFP containing enhanced green fluorescence protein gen（e EGFP）as control, and investigated the transducing rate through fluorescence to obtain the definite titre. Then TCa83 cell line was infected with AdCMV- TRAIL in proper titre, and TRAIL gene was detected by means of RT- PCR. After TCa83 cell line was infected with AdCMV- TRAIL and AdCMV- EGFP at day 1, 3, 5, 7, the activity of TCa83 cell line were evaluated by MTT and the apoptosis were detected by flow cytometer. Results Proper titre was of 1 000 particles/cell, and TCa83 cell line could be infected 100% in this titre. TRAIL gene was detected by RT- PCR after infected with AdCMV- TRAIL. The activity of TCa83 decreased in both groups, but the AdCMV- TRAIL group decreased more sharply than AdCMV- EGFP grou（p P<0.001）. Both AdCMV- TRAIL and AdCMV- EGFP could lead to apoptosis of TCa83 cells, but the AdCMV- TRAIL function stronger than AdCMV- EGFP. Especially there was remarkable statistic difference between two group（s P <0.000 1）. Conclusion AdCMV- TRAIL could effectively decrease the activity of TCa83 cell line and induce apoptosis.

Key words: squamous cell carcinoma, adenovirus vector, apoptosis gene, gene therapy