Abstract:

Objective To investigate the regulatory mechanism of parathyroid hormone-related protein（PTHrP） in proliferation and differentiation of chondrocytes of condyle in fetal mouse. Methods Chondrocytes of condyle in fetal mouse were separated and cultured in vitro, the influence of PTHrP on proliferation and differentiation was observed. Results After two weeks′ culture in 0.01 nmol/L, 0.1 nmol/L, 1 nmol/L, 10 nmol/L human PTHrP, there was significant difference in the number of cartilage nodule formed between experiment group and control group （P<0.05）, and there was no significant difference in 0.01 nmol/L group（P>0.05）. Alkaline phosphatase（ALP） activity was significantly intensified in experiment group and control group（P<0.05）. Meanwhile, it was found that this function of promotion was lessened after anti-PTHR antibody used. Conclusion It can be seen that PTHrP, via its receptor, can promote proliferation and differentiation of chondrocytes of condyle, which resemble its modulation mechanism in epiphyseal growth plate cartilage intramembrane in mandibule.

Key words: parathyroid hormone-related protein, parathyroid hormone receptor, condyle, cartilage