West China Journal of Stomatology ›› 2021, Vol. 39 ›› Issue (4): 398-404.doi: 10.7518/hxkq.2021.04.004

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Role of osteoclast differentiation in the occurrence of osteoarthritis of temporomandibular joint

Xiao Mian(), Hu Zhihui, Jiang Henghua, Fang Wei, Long Xing()   

  1. Dept. of Oral and Maxillofacial Trauma and Temporomandibular Joint Surgery, School and Hospital of Stomatology, Wuhan University, State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education (KLOBM), School and Hospital of Stomatology, Wuhan University, Wuhan 430000, China
  • Received:2020-07-14 Revised:2021-04-17 Online:2021-08-01 Published:2021-08-10
  • Contact: Long Xing E-mail:mingshore@whu.edu.cn;longxing@whu.edu.cn
  • Supported by:
    The National Natural Science Foundation of China(81771100)

Abstract: Objective

This study aimed to explore the role of osteoclast differentiation in the occurrence of temporomandibular joint osteoarthritis (TMJOA).


A mouse TMJOA model was constructed. Micro-CT was used to observe the changes in condylar bone during the development of TMJOA. Hematoxylin-eosin (HE) staining was used to observe the histological structure changes of the condyle of TMJOA mice. Tartrate resistant acid phosphatase (TRAP) staining was used to observe the presence of osteoclasts in TMJOA joint tissue. The synovial fluid of patients with TMJ-OA was collected to determine the effect on osteoclast differentiation.


Micro-CT revealed that the condyle of the TMJOA group had the most obvious damage in the second and third weeks, and the shape of the condyles also changed in a beak-like manner. HE staining showed that the condyle cartilage and subchondral bone structure of TMJOA mice were disordered in the second week. TRAP tissue staining showed that the number of osteoclasts of the TMJOA group obviously increased in the second week. Results of cell experiments showed that the number of osteoclast differentiation significantly increased after stimulation of synovial fluid from TMJOA patients, and the cell volume increased.


TMJOA animal models and TMJOA patient synovial cell experiments could induce osteoclast differentiation, indicating that osteoclast differentiation plays an important role in TMJOA occurrence.

Key words: temporomandibular joint osteoarthritis, synovial fluid, osteoclast, condyle, osteoclast differentiation

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