Abstract:

Objective To investigate the development of novel cell delivery carriers and evaluate the effects of platelet-rich plasma（PRP） and platelet-poor plasma（PPP） on cellular attachment and proliferation. Methods Rat gingival fibroblasts, periodontal ligament fibroblasts and osteoblasts were cultured in vitro. The biodegradable membranes were treated with PRP and PPP that were obtained from rat whole blood by gradient density centrifugation. The cellular attachment, proliferation and morphology on the untreated membranes and membranes treated with PRP and PPP were tested in vitro for 48 hours. Results There were significant differences between the untreated membranes and the membranes treated with PRP and PPP for three kinds of rat cells used in the experiment. There were no significant differences between those membranes treated with PRP and those treated with PPP. Three kinds of cells on the membranes treated with PRP were slight higher than those on the membranes treated with PPP. There were no significant differences in the same group for cells attachment. The rate of different cells proliferation on the untreated membranes was significant lower than those on the membranes treated with PRP and PPP at 1 hour and 48 hours after seeding. At the same time, the rate of different cells proliferation on the treated membranes at 48 hours was significant higher than those on the treated membranes at 1 hour. There was no significant differences in the same group for the rate of cells proliferation. Most of the cells attached on the membranes treated with PRP and PPP exhibited robust and elongated forms with numerous filopodial extensions. Conclusion The results showed that the biodegradable membranes treated with PRP and PPP could promote rat cell attachment and proliferation. It suggests that this novel technique will provide the experimental basis for the development of cell delivery carriers and periodontal regeneration.

Key words: ratgingivalfibroblasts, periodontalligamentfibroblasts, osteoblasts, attachment, proliferation