Abstract:

Objective　The purpose of this studywas to establishtransfergeneicTca8113 cell and evaluate the expression of hu- man endostatin (hES) gene in the cell colonein vitro.Methods　To transfect hES gene into Tca8113 cells, lipofectamin was complexed with plasmid encoding hES gene, and blasticidin S antibiotic was adopted to selectTca8113-hES cell clone. Immuno- histochemistry S-P method was adopted to detect the expression of hES in the transfergenic Tca8113 cellin vitro.Results　Trans- fected by hES, the transfergenic Tca8113 cells could grow and proliferate in RPMI-1640 culture medium containing blasticidin S antibiotic. The expression rate of hES reached 100%.Conclusion　hES gene can express in hES-transfected Tca8113 cellin vitro.

Key words: endostatin, gene transfer, cell, squamous cell carcinama of tongue