Human osteoprotegerin inhibits osteoclasts and promotes hydroxyapatite to repair the mandibular defects in ovariec-tomized rats

doi:10.7518/hxkq.2018.04.004

Abstract

Abstract:

Objective This study aims to investigate the effect of human osteoprotegerin (hOPG) gene-modified rat bone marrow mesenchymal stem cells (rBMSCs) combined with hydroxyapatite (HA) scaffolds on the repair of mandibular defects in ovariectomized rats. Methods rBMSCs were transfected with adenovirus carrying pDC316-hOPG-EGFP. The expression of hOPG and the inhibition of osteoclast function were detected by Western blot and bone-grinding experiment respectively. The model of mandibular bone defect in rats with osteoporosis was established; HA, untransfected rBMSCs-conjugated HA, and transfected rBMSCs-conjugated HA scaffolds were implanted into the mandibular bone defects. After six weeks, tartrate-resistant acid phosphatase staining and hematoxylin-eosin staining were used to observe the number of osteoclasts and repair of bone defect. Results Adenovirus carrying hOPG gene in vitro were successfully transfected into rBMSCs. The hOPG with anti-osteoclast activity was expressed by hOPG-rBMSCs, and rBMSCs expressing hOPG combined with HA scaffolds promoted mandibular defect repair. Conclusion rBMSCs transfected with hOPG gene inhibited the function of osteoc-lasts both in vitro and in vivo, and transfected rBMSCs com-bined with HA scaffolds promoted the repair of mandibular defects in rats with osteoporosis.

Key words: osteoprotegerin, bone defect, gene transfection, bone regeneration, osteoporosis

CLC Number:

R782

Feng Liao, Shibo Liu, Yao Liu, Hanghang Liu, Jian Hu, Xian Liu. Human osteoprotegerin inhibits osteoclasts and promotes hydroxyapatite to repair the mandibular defects in ovariec-tomized rats[J]. West China Journal of Stomatology, 2018, 36(4): 367-371.

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References 11

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