Analysis of salivary protease spectrum in chronic periodontitis

doi:10.7518/hxkq.2017.01.005

Abstract

Abstract:

Objective This study aimed to investigate the difference in salivary protease expression in patients with chro-nic periodontitis and normal individuals. Methods The stimulating saliva in patients with chronic periodontitis and normal individuals were collected. Protein chip technology was adapted to analyze salivary protease spectrum. Results Among the 34 proteases in the chip, disintegrin and metalloproteinase (ADAM)8, matrix metalloproteinase (MMP)-8, MMP-12, neprilysin/CD10, and uridylyl phosphate adenosine/urokinase showed a significantly increased concentration in the saliva of chronic periodontitis patients compared with those in the saliva of normal individuals (P<0.01). By contrast, the concentrations of ADAM9, a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)1, ADAMTS13, cathepsin B, E, L, V, X/Z/P, kallikrein 6, 7, 11, 13,MMP-9, proteinase 3, presenilin-1, and proprotein convertase 9 sharply decreased (P<0.05). Conclusion The results demonstrated that protease spectrum in the saliva of chronic periodontitis patients and normal indi-viduals significantly differed. Analysis of salivary protease spectrum is a potential clinical method to examine, diagnose, and monitor chronic periodontitis.

Key words: saliva, chronic periodontitis, protease spectrum

CLC Number:

R781.4

Qian Li, Xuedong Zhou, Yaping Fan, Tengyu Yang, Songtao Wu, Yu Yu, Jiao Chen, Ping Zhang, Yun. Feng. Analysis of salivary protease spectrum in chronic periodontitis[J]. West China Journal of Stomatology, 2017, 35(1): 37-42.

Figures/Tables 2

References 21

[1]	Van Nieuw Amerongen A, Bolscher JG, Veerman EC. Sali-vary proteins: protective and diagnostic value in cariology[J]. Caries Res, 2004, 38(3):247-253.
[2]	Chiappin S, Antonelli G, Gatti R, et al.Saliva specimen: a new laboratory tool for diagnostic and basic investigation[J]. Clin Chim Acta, 2007, 383(1/2):30-40.
[3]	Helmerhorst EJ, Oppenheim FG.Saliva: a dynamic proteome[J]. J Dent Res, 2007, 86(8):680-693.
[4]	Chaudhry AS.Comparing two commercial enzymes to esti-mate in vitro proteolysis of purified or semi-purified pro-teins[J]. J Anim Physiol Anim Nutr (Berl), 2005, 89(11/12):403-412.
[5]	Boudida Y, Gagaoua M, Becila S, et al.Serine protease in-hibitors as good predictors of meat tenderness: which are they and what are their functions[J]. Crit Rev Food Sci Nutr, 2016, 56(6):957-972.
[6]	Lima DP, Diniz DG, Moimaz SA, et al.Saliva: reflection of the body[J]. Int J Infect Dis, 2010, 14(3):e184-e188.
[7]	Armitage GC.Development of a classification system for periodontal diseases and conditions[J]. Ann Periodontol, 1999, 4(1):1-6.
[8]	Schipper RG, Silletti E, Vingerhoeds MH.Saliva as research material: biochemical, physicochemical and practical aspects[J]. Arch Oral Biol, 2007, 52(12):1114-1135.
[9]	Smith GA, Riedford K.Epidemiology of early childhood caries: clinical application[J]. J Pediatr Nurs, 2013, 28(4): 369-373.
[10]	Ng MW, Ramos-Gomez F, Lieberman M, et al.Disease management of early childhood caries: ECC collaborative project[J]. Int J Dent, 2014, 2014:327801.
[11]	Ng MW, Ramos-Gomez F.Disease prevention and mana-gement of early childhood caries[J]. J Mass Dent Soc, 2012, 61(3):28-32.
[12]	Guarino C, Legowska M, Epinette C, et al.New selective peptidyl di(chlorophenyl) phosphonate esters for visualizing and blocking neutrophil proteinase 3 in human diseases[J]. J Biol Chem, 2014, 289(46):31777-31791.
[13]	Rao NV, Wehner NG, Marshall BC, et al.Characterization of proteinase-3 (PR-3), a neutrophil serine proteinase. Struc-tural and functional properties[J]. J Biol Chem, 1991, 266(15):9540-9548.
[14]	Yang TY, Zhou WJ, Du Y, et al.Role of saliva proteinase 3 in dental caries[J]. Int J Oral Sci, 2015, 7(3):174-178.
[15]	蔡静莉, 杨凌峰, 王蓉, 等. ADAM家族研究进展[J]. 国外医学分子生物学分册, 2000, 22(4):197-201.
	Cai JL, Yang LF, Wang R, et al.Research progress of ADAM family[J]. J Med Mol Biol, 2000, 22(4):197-201.
[16]	郭丽丽, 郭胜利, 郭炜. ADAMTS家族在肿瘤中的研究进展[J]. 医学综述, 2015, 21(11):1975-1979.
	Guo LL, Guo SL, Guo W.Research progress of a disintegrin and metalloproteinase with thrombospondin family in tumor[J]. Med Recapitul, 2015, 21(11):1975-1979.
[17]	Inuzuka K, Ogata Y, Nagase H, et al.Significance of coex-pression of urokinase-type plasminogen activator, and matrix metalloproteinase 3 (stromelysin) and 9 (gelatinase B) in colorectal carcinoma[J]. J Surg Res, 2000, 93(2):211-218.
[18]	华小川, 赖文莉. 脑啡肽及其与牙疼痛关系的研究进展[J]. 国际口腔医学杂志, 2007, 34(3):197-199.
	Hua XC, Lai WL.Development of research of enkephalin in tooth pain[J]. Int J Stomatol, 2007, 34(3):197-199.
[19]	李磊, 李岩. Kallikrein 10的调控机制及其肿瘤临床应用的价值[J]. 中国肿瘤生物治疗杂志, 2014, 21(1):109-113.
	Li L, Li Y.Regulatory mechanisms and clinical application values of kallikrein 10 gene in cancer[J]. Chin J Cancer Bio-ther, 2014, 21(1):109-113.
[20]	Pan W, Zhu P, Huizhen YU.Advances in tissue kallikrein and TIMP on vascular remodeling[J]. Adv Cardiovasc Dis, 2014, 35(4):498-503.
[21]	王光平, 李明霞, 刘建国. 激肽释放酶4与釉质发生不全[J]. 国际口腔医学杂志, 2013, 40(4):537-539.
	Wang GP, Li MX, Liu JG.Kallikrein 4 and amelogenesis imperfecta[J]. Int J Stomatol, 2013, 40(4):537-539.