Abstract:

Objective To investigate the influence of carbon monoxide on the expression of adhesion moleculesstimulated by inflammatory cytokines on human gingival fibroblasts. Methods Human gingival fibroblasts were stimulatedwith 50 ng·mL-1 tumor necrosis factor（TNF）-α and 10 ng·mL-1 interleukin（IL）-1β concurrently in the presenceor absence of 500 μmol·L-1 carbon monoxide releasing molecule（CORM）. Expression of intercellular adhesion molecule（ICAM）-1, vascular cell adhesion molecule（VCAM）-1 at protein and mRNA level was examined by Western blot andreverse transcription polymerase chain reaction（RT-PCR）, respectively. Activity of transcription factor NF-κB was evaluatedby reporter gene assay. Results Expression of ICAM-1 and VCAM-1 on human gingival fibroblasts increaseddramatically after concurrent stimulation of TNF-α and IL-1β, while CORM inhibited the upregulation of ICAM-1 andVCAM-1. CORM decreased the activity of NF-κB stimulated by TNF-α and IL-1β. Conclusion Carbon monoxidecould be a promising way in treating of periodontitis.

Key words: human gingival fibroblasts, periodontitis, adhesion molecules, carbon monoxide