Abstract:

Objective To investigate the anti-tumor effect of monocytes/macrophages against Tca8113 cells and the secretion of vascular endothelial growth factor（VEGF） in acid microenvironment in vitro. Methods Peripheral blood mononuclear cells were extracted from healthy person′ s blood and cultivated to transform into monocytes/ macrophages. The monocytes/macrophages were cultured with Tca8113 in acid microenvironment（pH6.6 and pH6.8） and in normal microenvironment（pH7.2）. The anti-tumor effect of monocytes/macrophages against Tca8113 cells were examined by MTT assay. The expression of VEGF was detected by enzyme-link immunoassay（ELISA）. Results The anti -tumor effect of monocytes/macrophages against Tca8113 cells in acid environment was lower than in normal environment（P<0.05）. VEGF excreted by mononuclear/macrophage was significantly higher in acid microenvironment than in normal microenvironment. Conclusion Due to acid microenvironment inside tumor, the anti-tumor effect of monocytes/macrophages against tumor cell was decreased, but the secretion of VEGF was gradually increased. However, the function of monocytes/macrophages on anti-tumor need more research.

Key words: monocytes/mcrophages, tongue squamous cell carcinoma, acid, vascular endothelial growth factor