华西口腔医学杂志

• 基础研究 • 上一篇    下一篇

脉冲Nd:YAG激光加氟化钠对离体人牙釉质超微结构及氟离子分布水平的影响

张莉,刘鲁川   

  1. 第三军医大学大坪医院野战外科研究所,重庆400042
  • 收稿日期:2004-12-25 修回日期:2004-12-25 出版日期:2004-12-20 发布日期:2004-12-20
  • 通讯作者: 刘鲁川,Tel:023-68757738
  • 作者简介:张 莉(1970-),女,重庆人,主治医师,硕士
  • 基金资助:

    国家自然科学基金资助项目(39670784);重庆市科委课题 (2001-54-21)

In VitroEffect of Pulse Nd:YAG Laser Plus NaF on Ultrastructure and Fluoride Distribution of Enamel

ZHANG Li, LIULu-chuan   

  1. Daping Hospital & Research Institute ofSurgery,The Third Military Medical University,Chongqing400042, China
  • Received:2004-12-25 Revised:2004-12-25 Online:2004-12-20 Published:2004-12-20

摘要:

目的 探索脉冲Nd:YAG激光结合氟化钠使用的防龋机制。方法 选择24颗健康离体人恒前磨牙制成牙釉质标本。扫描电镜(SEM)、电子探针分别观察和分析激光加氟化钠组、激光组、氟化钠组、空白组(不处理)牙釉质表面超微结构及牙釉质内部氟离子分布水平。结果 ①SEM观察:激光加氟化钠组牙釉质表面玷污物全部去除,并呈部分熔融状和空泡状改变;牙釉质表面散在分布着细小的白色颗粒;熔融灶、裂纹、微孔呈白色高度阻射。 ②电子探针分析:激光加氟化钠组中氟离子平均重量百分比值增加明显,5μm处氟离子平均重量百分比值最高,明显高于空白组、激光组和氟化钠组(P<0·001);氟离子进入牙釉质的有效深度约15μm,高于氟化钠组的5μm。结论 脉冲Nd:YAG激光结合氟化钠作用后引起牙釉质表面部分熔融、改变牙釉质晶体结构、促进牙釉质对氟离子的吸收、去除牙面玷污层,因而具有防龋作用。

关键词: 脉冲Nd:YAG激光, 氟化钠, 牙釉质, 扫描电镜, 电子探针

Abstract:

Objective To investigate the effect of pulse Nd:YAG laser combined with NaF on caries resistance of human enamelin vitro.Methods 24 caries-free, constant premolares were cut into enamel mass. Surface ultrastructure and fluoride distribution of enamel in laser-NaFtreated group, lasertreated group, NaFtreated group and control groupwere observed by scan- ning electron microscope (SEM) and electron probe microanalyzer.Results ①SEMobservation: In laser-NaFtreated group, the smear layer was almost removed, the enamel surface showed partially melted-like and bubble-like appearance, diffuse white-small particles distributed on enamel surface, and melted position, microcrack and microhole appeared whitely blocking-reflection. ②Electron probe microanalyzer analysis: In enamel samples, the highest average Ci of F-and the hightest depth which F-en- tered in enamel were recorded in the laser-NaF treated group. The average Ci of F-in laser-NaF treated group was significantly increased compared with that in NaF-treated group or laser-treated group or control group (P<0·001).Conclusion The pulsed Nd:YAG laser combined with sodium fluoride could make the enamel surface partially melt, change the structure of the crystal, enhance the absorption of fluoride, and clear smear layer. This technology may resist the caries.

Key words: pulsed Nd:YAG laser, sodium fluoride, enamel, scanning electron microscope, electron probe microana- lyzer