关键词: 牵张成骨术, 下颌骨, 咀嚼肌, 酶组织化学

Abstract:

Objective:The purpose of this study was to study the masticatory muscles affected by distraction osteogenesis of the mandible.Methods:The distraction osteogenesis (DO) was applied to distract the leftmandible of 6 mongrel dogs thatwere divid- ed into three experimental groups. After different distraction phase and consolidation phase, the masseter and the digastric muscle were taken out. The specimenswere stained using hematoxylin/eosin and enzyme histochemistry. Afterwards, the specimenswere observed with a light microscope to study the morphologic changes of the muscles. The contents of enzyme in the different groups were measured by VIDAS.Results:The masseter showed consequently atrophy, but the digastric muscle showed a progress of his- tomorphologic reconstruction, including atrophy and hypertrophy. The changes of the contents of enzyme and histomorphology were identical in the masticatory muscles.Conclusion:The digastric muscle parallel to the vector of mandibular distraction adapts the distraction by the way of atrophy, regeneration and hypertrophy. And the contents of enzyme appear to decrease at the beginning, increase afterwards, and return to the normal level finally. But the masseter perpendicular to the vector of mandibular distraction shows consequent atrophy, and the contents of enzyme consequently decrease, which means the metabolism decrease.

Key words: distraction osteogenesis, mandible, masticatory muscles, enzyme histochemistry