华西口腔医学杂志

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功能矫治前伸下颌后大鼠血中骨钙素含量的昼夜变化规律

叶凌,李江宁,陈扬熙,罗颂椒   

  1. 610041 四川大学华西口腔医院正畸科
  • 收稿日期:2001-10-25 修回日期:2001-10-25 出版日期:2001-10-20 发布日期:2001-10-20
  • 基金资助:
    本课题为国家自然科学基金资助项目(编号39870751)

Changes of Osteocalcin in Serum of Young SD Rats after Functional Protrusion

Ye Ling, Li Jiangning, Chen Yangxi, et al   

  1. College of Stomatology,West China University of MedicalSciences
  • Received:2001-10-25 Revised:2001-10-25 Online:2001-10-20 Published:2001-10-20

摘要:

目的:研究生长发育期大鼠,在不同时间段戴用功能矫治器,其血中骨钙素含量的昼夜变化规律。方法:选用72只SD大鼠,随机等量分为对照组、白天12 h加力组和24 h加力组,戴用矫治器1周后按不同时间点处死大鼠,采用放射免疫法计测大鼠血中骨钙素含量的变化,应用宏观、微观分析法进行统计分析。结果:生长发育期的大鼠在自然状态下,其血中骨钙素存在着昼夜节律变化。戴用功能矫治器后,血中骨钙素的昼夜节律性并未消失, 且血中骨钙素的含量随着矫治器戴用时间的延长而增加,即全天24 h戴用功能矫治器,血中骨钙素均值升高幅度大于白天12 h戴用组和对照组。结论:生长发育期戴用功能矫治器时间越长,血中骨钙素的水平增高得越多,治疗效果越明显。

关键词: 功能矫治, 骨钙素, 昼夜节律

Abstract:

Objective:The aim of this study is to investigate temporal and spatial change patterns of osteocalcin in serum of young grow- ing SD rats, in conditions of different functional treatment time spans.Methods:Totally, 72 SD rats are randomly divided into 3 groups, including the control group, the 24-hourwearing group (wearing functional appliance 24 hours a day), and the 12-hour wearing group (wearing appliance for 12 hours in daylight). One week after the experiment, rats were sacrificed and osteocalcin level in serumwasmeasured by using radioimmunoassy. Date was processed using macroscopic and microscopic analysis.Results: Serum osteocalcin had circadian rhythm and the peakwas about12:00 in a day. Afterwearing the functional appliance, serum os- teocalcin level increased with treatment time, and serum osteocalcin level in the 24-hour wearing group rose more obviously than that in the 12-hourwearing group.Conclusion: Forthe functional appliance, better results can be achieved in the 24-hourwearing group.

Key words: functional treatment, osteocalcin, circadian rhythm