关键词: 白色假丝酵母菌, 毒力, 随机扩增多态性DNA, 多元回归

Abstract:

Objective To research the relationship between the virulence factors of Saccharomyces albicans（S.albicans） and the random amplified polymorphic DNA（RAPD） bands of them, and establish the regression model by multiple regression analysis. Methods Extracellular phospholipase, secreted proteinase, ability to generate germ tubes and adhere to oral mucosal cells of 92 strains of S.albicans were measured in vitro; RAPD-polymerase chain reaction（RAPDPCR） was used to get their bands. Multiple regression for virulence factors of S.albicans and RAPD-PCR bands was established. Results The extracellular phospholipase activity was associated with 4 RAPD bands: 350, 450, 650 and 1 300 bp（P<0.05）; secreted proteinase activity of S.albicans was associated with 2 bands: 350 and 1 200 bp（P<0.05）; the ability of germ tube produce was associated with 2 bands: 400 and 550 bp（P<0.05）. Conclusion Some RAPD bands will reflect the virulence factors of S.albicans indirectly. These bands would contain some important messages for regulation of S.albicans virulence factors.

Key words: Saccharomyces albicans, virulence, random amplified polymorphic DNA, multiple regression