关键词: ECV304细胞, 非白色假丝酵母菌, 增殖, 代谢产物

Abstract:

Objective  To evaluate the effects of non-Saccharomyces albicans metabolic products on the cell cycle distribution and proliferation of human umbilical vein endothelial cell ECV304 cells in vitro. Methods The parallel dilution supernatant of Saccharomyces tropicalis, Saccharomyces krusei and Saccharomyces glabrata were prepared, and 1, 4, 16-fold (s) diluted concentration and control group were set up. The line of human umbilical vein endothelial cell ECV304 was cultured in vitro and treated by non -Saccharomyce s albicans supernatant. The proliferous effect of ECV304 induced by non-Saccharomyces albicans supernatant after 24, 48, 72 h was detected by the methods of MTT, and the changes of cell density and cycle after 48 h were investigated by inverted microscope and flow cytometry. Results  At the 24th hour, all of the higher concentration (1 -folc) of non -Saccharomyce s albicans supernatant and the 4-folds diluted Saccharomyces krusei could promote ECV304 proliferation (P<0.05). After adding various non-Saccharo- myces albicans supernatant at 48th and 72th hour, Saccharomyces krusei supernatant and Saccharomyces glabrata supernatant significantly increased proliferation rate of ECV304, while Saccharomyces tropicalis supernatant group showed no significant change no matter which concentration was tested. At 48th hour after adding the non-Saccharomyces albicans supernatant, the ECV304 cells density treated by Saccharomyces krusei supernatant and Saccharomyces glabrata supernatant were significantly higher under the inverted microscope. The G0/G1 population of ECV304 cells decreased   while cell proliferation index (PI) increased after incubated with Saccharomyces krusei supernatant and Saccharomyces glabrata supernatant for 48 hours (P<0.05). Saccharomyce s tropicalis group showed no significant change (P>0.05) . Conclusion  The metabolic products of Saccharomyces krusei and Saccharomyces glabrata could induce proliferation of ECV304 cell, which suggests non-Saccharomyces albicans should be undergone more attention clinically in detection and treatment.

Key words: ECV304 cells, non-Saccharomyces albicans, proliferation, metabolic products