West China Journal of Stomatology ›› 2019, Vol. 37 ›› Issue (4): 378-383.doi: 10.7518/hxkq.2019.04.007

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Effect of long chain non-coding RNA H19 on the migration and invasion of oral cancer cells and its molecular mechanism

Junfang Zhao,Zhian Zha,Weihong Xie,Haibin Wang,Xinming Li,Qiang Sun(),Minglei Sun   

  1. Dept. of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
  • Received:2018-12-21 Revised:2019-02-19 Online:2019-08-01 Published:2019-08-23
  • Contact: Qiang Sun E-mail:85244275@qq.com
  • Supported by:
    The National Natural Science Foundation of China(81402231);The National Natural Science Foundation of Henan Province(182300410319)

Abstract:

Objective To investigate the effect of the long chain non-coding RNA H19 (lncRNA H19) on the invasion and migration of oral cancer cells and its related molecular mechanism. Methods The expression levels of lncRNA H19, miR-107, and cyclin-dependent kinase 6 (CDK6) in the immortalized oral epithelial cell line HIOEC and the oral cancer cell line CAL27 were detected by real-time quantitative polymerase chain reaction. CAL27 cells were transfected with siRNA H19, miR-107 mimics, pcDNA H19, or anti-miR-107, and the effects of H19 and miR-107 on the invasion and migration of cells were examined via Transwell assay. The TargetScan database predicted the targeting of H19, miR-107, and CDK6. Double luciferase reporter gene assay was performed to detect interactions among H19, miR-107, and CDK6. Western blot analysis was conducted to examine the effects of H19 and miR-107 on the protein level of the target gene CDK6. Results Compared with that in HIOEC cells, the expression of H19 was significantly increased in CAL27 cells (P<0.05). After transfection with siRNA H19, the expression of H19 decreased, and the invasion and migration ability of CAL27 cells were inhibited (P<0.05). H19 could bind specifically to the 3’-UTR of miR-107 to modulate the expression of miR-107. Compared with that in HIOEC cells, the expression of miR-107 significantly decreased in CAL27 cells (P<0.05). The expression of miR-107 increased after transfection with siRNA H19, and anti-mir-107 co-transfection could promote the invasion and migration ability of siRNA H19 in CAL27 cells (P<0.05). Compared with that in HIOEC cells, CDK6 expression significantly increased in CAL27 cells (P<0.05), and the expression level of the gene was co-regulated by H19 and miR-107 (P<0.05). Conclusion lncRNA H19 plays an important role in the development of oral cancer. It can regulate the invasion and migration of oral cancer cells by targeting the miR-107/CDK6 signaling axis.

Key words: oral cancer, long chain non-coding RNA H19, invasion, migration

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