华西口腔医学杂志 ›› 2017, Vol. 35 ›› Issue (5): 538-542.doi: 10.7518/hxkq.2017.05.018

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牙龈卟啉单胞菌第Ⅸ型分泌系统的研究进展

张尽美(), 赵蕾, 吴亚菲()   

  1. 口腔疾病研究国家重点实验室,国家口腔疾病临床医学研究中心,四川大学华西口腔医院牙周病科,成都 610041
  • 收稿日期:2017-04-02 修回日期:2017-07-05 出版日期:2017-10-01 发布日期:2017-10-01
  • 作者简介:

    张尽美,硕士,E-mail:qjzx.zjm.7086@163.com

  • 基金资助:
    国家自然科学基金(81371150);留学回国人员科研启动基金(2013-693-11-11)

Research progress on the type Ⅸ secretion system of Porphyromonas gingivalis

Jinmei Zhang(), Lei Zhao, Yafei Wu()   

  1. State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Dept. of Periodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2017-04-02 Revised:2017-07-05 Online:2017-10-01 Published:2017-10-01
  • Supported by:
    National Natural Science Foundation of China (81371150);Scientific Research Foundation for the Returned Overseas Chinese Scholars (2013-693-11-11)

摘要:

牙龈卟啉单胞菌第Ⅸ型分泌系统(T9SS)是新近发现的一种新型蛋白分泌系统,又被称为Por分泌系统(PorSS)。与以往发现的8个蛋白分泌系统不同,T9SS系统是一个仅存在于拟杆菌中的多蛋白复合体,其分泌的蛋白质N端均具有Sec依赖的Ⅰ型信号肽,C端均含有保守结构域(CTD)。牙龈卟啉单胞菌T9SS包括了内膜、外膜、细胞质以及细胞周质等重要组成部分的蛋白质,至少包括34种包含CTD的蛋白,这些蛋白参与调控的相关毒力因子包括牙龈素、菌毛、脂多糖、HBP35、CPG70蛋白和蛋白质-肽基精氨酸脱亚氨酶等。这些包含CTD的毒力蛋白通过T9SS转定位在细胞外膜上,然后释放至胞外,从而对牙周组织起破坏作用。本文对牙龈卟啉单胞菌T9SS的研究进展作一综述,以便于深入理解该菌的毒力机制。

关键词: 牙龈卟啉单胞菌, 第9型分泌系统, C端保守结构域, 牙龈素

Abstract:

In recent years, the study found that Porphyromonas gingivalis type Ⅸ secretion system (T9SS) is a novel protein secretion system, also known as Por secretion system (PorSS). Unlike the eight protein secretion systems found in the past, the system is a polyprotein complex found only in Bacteroides. The secreted proteins have both N- and C-terminus, where the former includes Sec-dependent typeⅠsignals peptide, and the latter contains conserved domains (C-terminal conserved domain, CTD). Porphyromonas gingivalis T9SS includes proteins such as intima, outer membrane, cytoplasm, and cell cycle, including at least 34 proteins containing CTD. Porphyromonas gingivalis T9SS is involved in regulating associated virulence factors including gingivin, fimbriae, lipopolysaccharide, HBP35, CPG70 protein and peptidyl-arginine deiminase. These CTD-containing virulence proteins are localized by T9SS and then released to the extracellular domain, thereby destroying periodontal tissue. Therefore, this review summarizes the research progress on the T9SS of Porphyromonas gingivalis.

Key words: Porphyromonas gingivalis, type Ⅸ secretion system, C-terminal conserved domain, gingipains

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