华西口腔医学杂志

• 基础研究 • 上一篇    下一篇

丹酚酸B对人牙周膜细胞成骨分化的影响

马玥1,2 任嫒姝1,2 付钢3   

  1. 1.重庆医科大学附属口腔医院正畸科；2.口腔疾病与生物医学重庆市重点实验室；3.重庆市高校市级口腔生物医学工程重点实验室，重庆401147
  • 收稿日期:2016-01-06 修回日期:2016-07-12 出版日期:2016-10-01 发布日期:2016-10-01
  • 通讯作者: 任嫒姝，副教授，博士，E-mail：rasras@163.com
  • 作者简介:马玥，硕士，E-mail：yaotong6@hotmail.com
  • 基金资助:

    重庆市渝中区科委基金（20140128）；重庆市卫生局基金资助项目（2011-2-180）；2013年重庆高校创新团队建设计划资助项目（KJTD201314）

Effects of salvianolic acid B on osteogenic differentiation of human periodontal ligament cells

Ma Yue1,2, Ren Aishu1,2, Fu Gang3.   

  1. 1. Dept. of Orthodontics, Stomatological Hospital of Chongqing Medical University, Chongqing 401147, China; 2. Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing 401147, China; 3. Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China
  • Received:2016-01-06 Revised:2016-07-12 Online:2016-10-01 Published:2016-10-01
  • Contact: Ren Aishu, E-mail: rasras@163.com.
  • Supported by:

    Scientific and Technological Research Program of Chongqing Yuzhong (20140128); Project Supported by Health Bureau of Chongqing (2011-2-180); Program for Innovation Team Building at Institutions of Higher Education in Chongqing in 2013 (KJTD201314).

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摘要：

目的 探讨中药丹参主要活性成分丹酚酸B（SalB）对人牙周膜细胞（hPDLCs）成骨分化的影响。方法 取第3代hPDLCs进行实验，运用甲基噻唑基四唑（MTT）法检测不同浓度丹酚酸B对hPDLCs增殖活性影响，通过检测碱性磷酸酶（ALP）活性、矿化结节染色、骨钙素（OCN）mRNA表达来探讨丹酚酸B对hPDLCs成骨分化的影响。结果 丹酚酸B对hPDLCs增殖活性无影响。当丹酚酸B浓度为0.5、1、5 μmol·L-1时均能增加hPDLCs的ALP活性和OCN mRNA表达，与OIM组比较差异有统计学意义（P<0.05）；且当丹酚酸B浓度为0.5、1、5 μmol·L-1时hPDLCs形成矿化结节数量明显高于OIM组。结论 适宜浓度的丹酚酸B能有效促进hPDLCs成骨分化。

关键词: 丹酚酸B, 人牙周膜细胞, 成骨分化, 碱性磷酸酶活性, 矿化结节

Abstract:

Objective  This study investigated the effects of salvianolic acid B (Sal B), a major bioactive component of the Chinese medicine salvia miltiorrhiza, on osteogenic differentiation of human periodontal ligament cells (hPDLCs). Methods Third passage PDLCs were used in this experiment. Methyl thiazolyl tetrazolium (MTT) method was employed to observe the effects of different Sal B concentrations on proliferation activity of hPDLCs. Alkaline phosphatase (ALP) activity and mineralization capability were measured, and mRNA expression of osteocalcin (OCN) was detected to investigate the effects of Sal B on osteogenesis of hPDLCs. Results  Sal B did not influence the viability of hPDLCs. The ALP activity and OCN mRNA expression levels of hPDLCs were both significantly improved (P<0.05) under treatment with different Sal B concentrations (0.5, 1, and 5 μmol·L−1) compared with those in OIM group. Moreover, the number of mineralized nodules formed by hPDLCs were considerably higher under treatment with different Sal B concentrations (0.5, 1, and 5 μmol·L−1) than that in the OIM group. Conclusion  Appropriate Sal B concentration can improve the osteogenic differentiation of hPDLCs.

Key words: salvianolic acid B, human periodontal ligament cells, osteogenic differentiation, alkaline phosphatase activity, mineralized nodules

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