Abstract:

Objective To evaluate the effects of mechanical stress on the formation and expression of core binding factor α1（Cbfα1） in MG-63 cells cultured on titanium in vitro. Methods MG-63 cells cultured on the titanium were subjected to a centrifugal force（2.205 N） 15 min per 4 hours and collected after 4, 8 and 12 hours. The formation and expression of Cbfα1 were examined by immunofluorescence staining and reverse transcription-polymerase chain reaction（RT-PCR）. Results Both the cells with or without centrifugal force created the fluorescence in the nucleus and the immunofluorescence intensity of Cbfα1 in MG-63 cells with centrifugal force were higher than those without centrifugal force（P<0.05）. Meanwhile, both the cells with or without centrifugal force expressed the mRNA of Cbfα1 and the relative mRNA level of Cbfα1 in MG-63 cells with centrifugal force were higher than those without centrifugal force, and the differences were great significant（P<0.05）. Conclusion Mechanical stress are beneficial to the formation and expression of Cbfα1.

Key words: mechanicalstress, titanium, osteoblast, corebindingfactorα1