West China Journal of Stomatology ›› 2018, Vol. 36 ›› Issue (4): 404-409.doi: 10.7518/hxkq.2018.04.011

• Orginal Article • Previous Articles     Next Articles

Role and mechanism of Galectin-3 gene in proliferation, invasion, and apoptosis of oral squamous cell carcinoma

Zheng Fang(), Feng Qiu, Junfang Zhao(), Qiang Sun, Bin Qiao, Guanghui Li, Xinming Li   

  1. Dept. of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
  • Received:2017-07-27 Revised:2018-04-10 Online:2018-08-01 Published:2018-08-01
  • Supported by:
    The National Natural Science Foundation of China (8140100298).


Objective The effect and mechanism of Galectin-3 gene expression on proliferation, invasion, and apoptosis of oral squamous cell carcinoma (OSCC) were investigated. Methods Reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting were used to detect the mRNA and protein of Galectin-3 gene in OSCC. OSCC Tca8113 was divided into control, negative control, and Galectin-3 transfection groups. Western blotting was used to detect the expression of Galectin-3, matrix metalloproteinase (MMP)-2, MMP-9, Cleaved Caspase-3, β-catenin, and Cyclin D1 protein after transfection for 48 h in each group. Cell proliferation was detected by CCK8. Cell invasion ability was detected by using a Transwell chamber. Cell apoptosis was detected by flow cytometry. Results The mRNA and protein expression levels of Galectin-3 gene in OSCC were significantly higher than those in adjacent tissues (P<0.01). Galectin-3 protein expression in Tca8113 cells significantly decreased after RNA interference. Cell survival rate and invasion as well as MMP-2, MMP-9, β-catenin, and Cyclin D1 protein expression were significantly lower than the blank group. Apoptosis rate and Cleaved Caspase-3 protein expression were sig-nificantly higher than the control group (P<0.01). Conclusion Inhibition of Galectin-3 gene expression in OSCC can signi-ficantly reduce the proliferation and invasion of cancer cells and induce apoptosis. The mechanism is related to downregulation of the Wnt/β-catenin signaling pathway.

Key words: Galectin-3 gene, oral squamous cell car-cinoma, proliferation, invasion, apoptosis

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