Abstract:

Objective Through a simulation of interstitial fluidpressure (IFP), we developed an in vitro model to explore the change law of biological characteristics of adenoid cystic carcinoma (ACC) under different IFP. Methods A pressure cooker was refitted into a controllable pressure device. Cultured ACC-2 cells were subdivided into different groups, namely, negative control (untreated ACC-2) and experimental group (stressed for 3, 6, 12, 24 h under pressure of 7.551, 7.649, 7.747kPa). CCK-8 and immunofluorescence of Ki67 were used to reflect proliferation ability. Transwell chamber assay was performed to observe the invasion ability of cells. Results The proliferation ability was positively correlated with treatment time, and the peak value was obtained after the cells were subjected to 7.649 kPa of stress for 24h. The invasion ability of ACC-2 cells was upregulated under stress. Conclusion We successfully developed an in vitro model of IFP and found that highIFP canstimulate cell proliferation abilityandupregulate invasion ability.

Key words: interstitial fluid pressure, adenoid cystic carcinoma, pressure, cell proliferation, cell invasion ability