Abstract:

Objective To investigate the anti- proliferation effect of tyrosine protein kinase inhibitor, Genistein, on human salivary adenoid cystic carcinoma cell line SACC - 83, and its effect on Survivin expression. Methods SACC- 83 cells were treated with different concentration Genistein for different time, cell survival rate was calculated with MTT assay, apoptosis was detected with flow cytometry, the expression of Survivin was quantitatively analyzed by Western blotting and FluorChem V2.0 software. Results When treated with Genistein of certain concentration for certain time, SACC- 83 cell growth was significantly inhibited. With the increase of concentration and elongation of acting time, the inhibitory effects increase. Treated with 220 μmol/L Genistein for 72 hours, SACC- 83 cell growth was significantly inhibited, cell apoptosis was induced（P<0.01）, and the expression of Survivin decreased. Conclusion Genistein inhibits the growth of human salivary adenoid cystic carcinoma cell line SACC- 83, and induces cell apoptosis; the decrease of Survivin expression may be one of the mechanisms of Genistein inducing apoptosis.

Key words: Genistein, salivary adenoid cystic carcinoma, Survivin, apoptosis