Abstract:

Objective　To study the biocompatibility of bone engineering scaffolds designed and fabricated by CAD and Rapid Prototyping techniques.Methods　Infant rat calvarias osteoblastswere isolated and expandedin vitroand the cells (2nd passage) were seeded onto scaffolds with porosity 80%,90%,95% at a density of 2·06×109/L. Cell adhesion number and morphology were measured with SEM after 4 days, 10 days co-culture.Results　①The osteoblasts′adhesion amounts increased with culture time in three porosity group (P<0·05), but the increase were different among three groups, 80% group was 0·35×105, 90% group was 2·84×105;②Through SEM observations,it showed that osteoblasts adhered to all scaffolds well.Conclusion　The scaffolds designed and fabricated by CAD and rapid prototyping own a good cellular biocompatibility. The results suggest the feasi- bility of using such scaffold fabricating method for bone tissue engineering research and clinical therapy.

Key words: bone tissue engineering, osteoblast adhesion, rapid prototype, cellular biocompatibility