Abstract:

Objective　To studythe effect of phosphorothioate antisense Bcl-xLoligodeoxynucleotides on apoptosis and thermo- sensitivity of BcaCD885 cells.Methods　After phosphorothioate antisense Bcl-xL oligodeoxynucleotides were tranfected into BcaCD885 cells. The characteristics of apoptotic cellswere evaluated bymorphological observation andTUNELstaining. Apoptotic rate and Bcl-xL protein expression were analyzed with flow cytometry. The influence of phosphorothioate antisense Bcl-xL oligode- oxynucleotides on apoptotic rate of BcaCD885 cells induced by hyperthermiawith 43℃40 minwas also examined through flow cy- tometry.Results　The BcaCD885 cells transfected with phosphorothioate antisense Bcl-xL oligodeoxynucleotides displayed apop- totic morphological features. The Bcl-xL protein expression level of these cells was down-regulated significantly compared with the controlled group (P<0.05). The apoptotic rate of these cells induced by hyperthermia was increased significantly compared with the controlled group (P<0.05).Conclusion　Phosphorothioate antisense Bcl-xL oligodeoxynucleotides can induce apoptosis and improve thermosensitivity of BcaCD885 cells.

Key words: antisense oligodeoxynucleotides, Bcl-xL, BcaCD885 cells, apoptosis, thermosensitivity