Abstract:

Objective To explore the expression of glucose transporter（GLUT）-1 and insulin receptor（IR） α1 in osteoblast obtained from diabetic rats’mandibles. Methods The expression of GLUT-1 and IR α1 of diabetic and control groups were measured by reverse transcription polymerase chain reaction, Western blot and immunohistochemistry stain. Results The mRNA expressions of GLUT-1 and IR α1 of diabetic group were significantly higher than control group（P<0.05）. The protein expressions of GLUT-1 and IR α1 were similar to the mRNA expressions. Conclusion Osteoblasts obtained from diabetic rats’mandibles keep the adaptation changes to hyperglycemia and hypoinsulinemia, which may contribute to their dysfunction.

Key words: diabetes mellitus, mandible, osteoblast, glucose transporter, insulin receptor