Abstract:

Objective To investigate the proliferation effects of arsenic trioxide（As2O3） on salivary adenoid cystic carcinma-2（ACC-2） cells in vitro and to study the role of Survivin on the apoptosis of ACC-2 induced by As2O3. Methods ACC-2 cells were treated with different concentration of As2O3 for different time. The inhibitory effects on cell′s viability were assayed with methyl thiazolyl tetrazolium（MTT） test. Apoptosis was determined by flow cytometry. The expression of Survivin mRNA and protein were investigated by reverse transcription -polymerase chain raction （RT-PCR） and Western blot analysis respectively. Results Cell viability after As2O3 treatment was markedly suppressed and exhibited as a dose- and time-dependent pattern. The apoptotic index showed the similar trend. The results of RT-PCR revealed gene expression of Survivin was suppressed significantly. Through Western blot analysis, a negative correlation between concentration and amount of protein product of Survivin was determined. Conclusion As2O3 might markedly suppressed ACC-2 cell′s viability in vitro. The inhibition of Survivin gene expression may play a critical role on ACC-2 cell apoptosis induced by As2O3.

Key words: arsenic trioxide, adenoid cystic carcinoma, apoptosis