Abstract:

Objective To study the cultural method and identification of human umbilical vein endothelial cells （HUVECs）, and investigate the expression of tyrosine kinase-2 with immunoglobulin-like and epidermal growth factor homology domains（Tie-2） in HUVECs. Methods HUVECs were isolated from umbilical veins by the technique of irrigative digestion, and were cultivated in plates. The cells were identified by Ⅷ monoclonal antibody. Tie-2 mRNA and protein were detected by reverse transcription-polymerase chain reaction （RT-PCR） and SABC immunocytochemistry. Results HUVECs could adhere to the plates completely after 24 hours, and confluence a monolayer 4-5 days later. The band of Tie-2 mRNA was obviously and the expression of Tie-2 protein was strongly positive by immunocytochemistry in HUVECs. The positive rate was over 85％. Conclusion Highly purified endothelial cells were isolated. And there were overexpression of Tie-2 in HUVECs.

Key words: human umbilical vein endothelial cells, tyrosine kinase-2 with immunoglobulin-like and epidermal growth factor homology domains, tumor