关键词: 磁性附着体, 静磁场, 成骨细胞, 增殖活性, 细胞周期, 细胞凋亡

Abstract:

Objective To investigate the effects of the static magnetic field（SMF） generated by dental magnetic attachments on osteoblastic proliferations, cell cycle distribution, and apoptosis ratio. Methods By simulating those of the closed- field, the closure process and the open- field Magnedisc 800 magnetic attachments respectively, the in vitro cultured rat osteoblasts were exposed continuously to 12.5, 125, 250 mT SMF. The effects of the SMF on the proliferation of the cells were examined. MTT colorimetry test was performed to detect the effect of the SMF on the vitalities of cells. Flow cytometry was utilized to analyze the cell cycles and cell apoptosis rates. Results The SMF exposure didn′t change the vital osteoblasts number, the cell cycle distribution and proliferation activities of osteoblasts. The cell apoptosis situation were not observed statistical differences. Conclusion No matter the closed- field, the closure process and the open- field magnetic attachments respectively, continuous simulating SMF- stimulation of magnetic attachments couldn′t change osteoblasts proliferation activity, cell cycle distribution, and apoptosis ratio.

Key words: magnetic attachment, static magnetic field, osteoblasts, proliferation, cell cycle distribution, cell apoptosis