关键词: 微弧氧化, 激光共聚焦显微镜, 成骨细胞, 细胞骨架

Abstract:

Objective  This study aimed to evaluate the effects of the microarc oxidation surface on cell morphology and cytoskeleton. Methods  Pure titanium with a diameter of 15 mm and a thickness of 1 mm was divided into four groups: grooved surfaces (group G); sandblasted surfaces (group SB); grooved microarc oxidation surfaces (group GMAO); and sandblasted microarc oxidation surfaces (group SBMAO). Osteoblast cells were cultured in each group. The morphology and proliferation of the cells on the titanium surface were observed by scanning electron microscope (SEM). The cytoskeleton was evaluated by laser scanning confocal microscope (LSCM). Results  The osteoblasts were inoculated after 12 h; these osteoblasts then spread along the surface of the titanium plate. In GMAO and SBMAO groups, the osteoblasts converged in the hole. The actin fibers in each group were clearly visible. In particular, the actin fibers in GMAO and SBMAO groups were arranged in parallel and formed bundles that extended into the holes. Conclusion  After microarc oxidation, the tita-nium surface significantly affected the morphology and cytoskeleton of osteoblasts.

Key words: microarc oxidation, laser scanning confocal microscope, osteoblasts, cytoskeleton