硅酸钙的缺陷改性及其在口腔抑菌和牙齿再矿化的应用研究

doi:10.7518/hxkq.2025.2025004

华西口腔医学杂志 ›› 2025, Vol. 43 ›› Issue (5): 648-659.doi: 10.7518/hxkq.2025.2025004

• 基础研究 • 上一篇

硅酸钙的缺陷改性及其在口腔抑菌和牙齿再矿化的应用研究

胡媛媛¹(), 张书颜¹, 张键华¹, 罗红蓉², 李运峰³, 张静⁴(), 陈显春¹()

^1.四川大学高分子科学与工程学院，成都 610065
^2.四川大学国家生物医学材料工程研究中心，成都 610065
^3.口腔疾病防治全国重点实验室国家口腔医学中心国家口腔疾病临床医学研究中心四川大学华西口腔医院正颌及关节外科，成都 610041
^4.四川大学建筑与环境学院，成都 610065

收稿日期:2025-01-03 修回日期:2025-02-27 出版日期:2025-10-01 发布日期:2025-10-21
通讯作者: 张静,陈显春 E-mail:2022223090012@stu.scu.edu.cn;zjing428@163.com;chenxianchun@scu.edu.cn
作者简介:胡媛媛，硕士，E-mail：2022223090012@stu.scu.edu.cn
基金资助:
国家重点研发计划(2022YFB3804500);国家自然科学基金(52473312)

Defect modification of calcium silicate and its application in oral bacteriostasis and tooth remineralization

Hu Yuanyuan¹(), Zhang Shuyan¹, Zhang Jianhua¹, Luo Hongrong², Li Yunfeng³, Zhang Jing⁴(), Chen Xianchun¹()

^1.College of Polymer Science and Engineering, Sichuan University, Chengdu 610065, China
^2.National Biomedical Materials Engineering Research Center, Sichuan University, Chengdu 610065, China
^3.State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases & Dept. of Orthognathic and Temporomandibular Joint Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
^4.College of Architecture and Environment, Sichuan University, Chengdu 610065, China

Received:2025-01-03 Revised:2025-02-27 Online:2025-10-01 Published:2025-10-21
Contact: Zhang Jing,Chen Xianchun E-mail:2022223090012@stu.scu.edu.cn;zjing428@163.com;chenxianchun@scu.edu.cn
Supported by:
National Key Research and Development Program(2022YFB3804500);National Natural Science Foundation of China(52473312)

摘要/Abstract

摘要：

目的对硅酸钙（CSO）改性赋予光热抑菌性能，评估其在口腔抑菌和牙齿再矿化方面的应用潜力。方法采用缺陷工程改性策略，通过硼氢化钠（NaBH₄）热还原工艺将氧空位引入CSO，制备4组缺陷改性CSO-T样品（CSO-300、CSO-400、CSO-500、CSO-600）。通过扫描电子显微镜（SEM）、X 射线衍射仪、X射线光电子能谱仪、紫外近红外吸收光谱、红外热像仪对样品进行检测，选取光热性能最好的粉末样品和最适合的材料浓度（CSO-500，500 μg/mL）进行后续实验。采用高分辨率透射电子显微镜分析样品的微观晶体结构和形貌，MTT法及Calcein-AM/PI活/死细胞染色检测评估对人口腔角质形成细胞的毒性及相容性。选择大肠杆菌、金黄色葡萄球菌进行光热抑菌实验，评价体外抑菌性能。利用SEM、能谱仪、显微维氏硬度计评价材料体外诱导离体牙再矿化能力。结果氧空位缺陷改变了CSO的晶型和晶格间距，拓宽了光吸收范围，赋予了良好的近红外响应光热转换能力。缺陷改性后的CSO能够促进羟磷灰石在牙齿表面的生成，实现牙齿再矿化，提升牙齿的硬度，并且具有光热抑菌特性且无细胞毒性。结论氧空位缺陷改性黑色硅酸钙兼具促牙齿再矿化、光热抑菌等功能，将其与红外发光牙刷结合使用，可简单有效治疗过量饮用碳酸饮料等日常不良生活习惯导致的牙釉质侵蚀和口腔细菌相关疾病，有望通过日常清洁达到牙齿再矿化和口腔抑菌协同治疗的效果。

关键词: 牙齿再矿化, 硅酸钙, 光热效应, 抑菌, 氧空位

Abstract:

Objective Calcium silicate (CSO) is modified to give it photothermal antibacterial properties. Its application potential in tooth mineralization and oral antibacterial is evaluated. Methods Based on defect-engineering modification strategy, a series of CSO-T samples (CSO-300, CSO-400, CSO-500, CSO-600) was obtained by introducing oxygen vacancy into CSO through thermal reduction using sodium borohydride. The samples were tested using scanning electron microscopy (SEM), X-ray diffraction, X-ray photoelectron spectroscopy, ultraviolet near-infrared absorption spectroscopy, and infrared thermography. The powder samples with the best photothermal performance and the most suitable material concentration (CSO-500, 500 μg/mL) were selected for subsequent experiments. High resolution transmission electron microscopy was used to analyze the microstructure and morphology of the sample, and MTT assay and Calcein AM/PI live/dead cell staining were used to evaluate the toxicity and compatibility of the sample to human oral keratinocytes. Escherichia coli and Staphylococcus aureus were selected for photothermal antibacterial experiments to evaluate their in vitro antibacterial performance. SEM, energy dispersive spectrometer, and micro Vickers hardness tester were used to evaluate the ability of materials to induce in vitro remineralization of detached teeth. Results Oxygen vacancies changed the crystal type and lattice spacing of CaSiO₃, broadened the light-absorption range, and gave it a good photothermal conversion ability in response to near infrared. Invitro experiments showed that the modified CaSiO₃ could promote the formation of hydroxyapatite on the tooth surface, thereby promoting the remineralization of teeth and improving the teeth hardness. Moreover, it had photothermal antibacterial properties and no cytotoxicity. Conclusion Defect-modified black calcium silicate has multiple functions, such as promoting tooth remineralization and photothermal bacteriostatic. When combined with the infrared luminescent toothbrush, it can simply and effectively treat tooth enamel erosion and oral bacteriostatic diseases caused by the excessive consumption of carbonated beverages and other daily bad living habits. This combination is expected to achieve the synergic treatment effect of tooth remineralization and oral bacteriostatic through daily cleaning is expected.

Key words: tooth remineralization, calcium silicate, photothermal effect, bacteriostasis, oxygen vacancy

中图分类号:

R783.1

胡媛媛, 张书颜, 张键华, 罗红蓉, 李运峰, 张静, 陈显春. 硅酸钙的缺陷改性及其在口腔抑菌和牙齿再矿化的应用研究[J]. 华西口腔医学杂志, 2025, 43(5): 648-659.

Hu Yuanyuan, Zhang Shuyan, Zhang Jianhua, Luo Hongrong, Li Yunfeng, Zhang Jing, Chen Xianchun. Defect modification of calcium silicate and its application in oral bacteriostasis and tooth remineralization[J]. West China Journal of Stomatology, 2025, 43(5): 648-659.

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项目	酸蚀前牙齿	酸蚀后牙齿	CSO-500处理后酸蚀牙	CSO处理后酸蚀牙
O/%	78.47	90.43	66.24	80.52
P/%	9.64	4.95	13.34	8.99
Ca/%	11.89	4.62	20.42	10.49
Ca/P	1.23	0.93	1.53	1.17

硅酸钙的缺陷改性及其在口腔抑菌和牙齿再矿化的应用研究

Defect modification of calcium silicate and its application in oral bacteriostasis and tooth remineralization

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