选择性激光烧结多孔钛种植体及其性能研究

doi:10.7518/hxkq.2018.05.013

华西口腔医学杂志 ›› 2018, Vol. 36 ›› Issue (5): 532-538.doi: 10.7518/hxkq.2018.05.013

选择性激光烧结多孔钛种植体及其性能研究

魏霆¹(),张欣蔚²,孙惠强^1,³(),毛梦芸⁴

^1. 山东大学口腔医学院修复科,济南 250012
^2. 安徽医科大学第二附属医院口腔科,合肥 230601
^3. 山东省口腔生物医学重点实验室,济南 250012
^4. 浙江大学医学院附属口腔医院综合科,杭州 310006

收稿日期:2018-01-02 修回日期:2018-07-08 出版日期:2018-10-01 发布日期:2018-10-18
作者简介:魏霆,硕士,E-mail： 2440941680@qq.com
基金资助:
山东省自然科学基金(ZR2014HM053)

Selective laser sintering and performances of porous titanium implants

Ting Wei¹(),Xinwei Zhang²,Huiqiang Sun^1,³(),Mengyun Mao⁴

^1. Dept. of Prosthodontics, College of Stomatology, Shandong University, Jinan 250012, China
^2. Dept. of Sto-matology, The Second Hospital of Anhui Medical University, Hefei 230601, China
^3. Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, China
^4. Dept. of Prosthodontics, Affiliated Stomatology Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China

Received:2018-01-02 Revised:2018-07-08 Online:2018-10-01 Published:2018-10-18
Supported by:
The National Natural Science Foundation of Shandong Province(ZR2014HM053)

摘要/Abstract

摘要：

目的分析选择性激光烧结（SLS）多孔钛种植体的机械性能及生物相容性,探讨其与壳聚糖（CS）/羟磷灰石（HA）复合涂层结合后的促骨结合作用。方法制备Ti6Al4V试件,部分试件表面进行CS/HA涂层处理;对试件进行扫描电子显微镜观察和机械性能检测;体外培养MC3T3-E1细胞,进行活/死细胞染色、甲基噻唑基四唑（MTT）及碱性磷酸酶（ALP）水平检测;将柱状螺纹种植体植入兔股骨髁部,分析其体内生物学性能。结果试件准弹性梯度随孔隙率增大而减小,孔隙率为30%时与皮质骨接近,70%时与松质骨接近;试件具有良好的生物相容性。复合CS/HA涂层后可促进MC3T3-E1细胞增殖、分化,有利于骨组织长入孔隙,形成良好的骨结合。结论多孔钛种植体具有良好的机械性能和生物相容性,与CS/HA涂层结合后具有骨诱导性,利于形成稳定的骨结合。

关键词: 选择性激光烧结, 钛种植体, 壳聚糖/羟磷灰石涂层, 生物相容性

Abstract:

Objective This work aims to analyze the mechanical properties and biocompatibility of porous titanium (Ti) implants fabricated by selective laser sintering (SLS) and investigate the promotion of osseointegration by porous titanium implant combined with chitosan (CS)/hydroxyapatite(HA) composite coating. Methods Ti6Al4V specimens were prepared, and CS/HA composite coating was fabricated on the surface of a portion of the specimens. The mechanical properties of the samples were observed by scanning electron microscope. MC3T3-E1 cells were cultured in vitro, and their biological properties in vitro were analyzed using live and dead viability cell staining method, methyl thiazolyl tetrazolium (MTT) staining, and alkaline phosphatase (ALP) level detection. The thread implant specimens were implanted in the femoral condyle of rabbits, and biological performance was evaluated in vivo. Results Quasi-elastic gradient of porous specimens decreased with in-creasing porosity, and the quasi-elastic gradient were close to cortical and cancellous bone when the porosities were 30% and 70%. The specimens showed good biocompatibility. Combined with CS/HA coating, the implants promoted the proliferation and differentiation of MC3T3-E1 cells and facilitated the entry of bone tissue into pores and good osteogenesis. Conclusion The porous titanium implant exhibited favorable mechanical properties and biocompatibility. Combined with CS/HA coating, the implant exhibited bone inducibility, which leads to stable osteogenesis.

Key words: selective laser sintering, titanium implant, chitosan/hydroxyapatite composite coating, biocompatibility

中图分类号:

R783.2

魏霆,张欣蔚,孙惠强,毛梦芸. 选择性激光烧结多孔钛种植体及其性能研究[J]. 华西口腔医学杂志, 2018, 36(5): 532-538.

Ting Wei,Xinwei Zhang,Huiqiang Sun,Mengyun Mao. Selective laser sintering and performances of porous titanium implants[J]. West China Journal of Stomatology, 2018, 36(5): 532-538.

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参考文献 12

[1]	Torres Y, Trueba P, Pavón J , et al. Designing, processing and characterisation of titanium cylinders with graded poro-sity: an alternative to stress-shielding solutions[J]. Mater Design, 2014,63(2):316-324. doi: 10.1016/j.matdes.2014.06.012 URL
[2]	Niinomi M . Recent research and development in titanium alloys for biomedical applications and healthcare goods[J]. Sci Technol Adv Mater, 2003,45:445-454.
[3]	Deckard C, Beaman JJ . Process and control issues in selec-tive laser sintering[C]. Chicago: ASME, 1988,33:191-197.
[4]	Kim BS, Kim JS, Chung YS , et al. Growth and osteogenic differentiation of alveolar human bone marrow-derived me-senchymal stem cells on chitosan/hydroxyapatite composite fabric[J]. J Biomed Mater Res A, 2013,101(6):1550-1558.
[5]	Wu SL, Liu XM, Yeung K , et al. Surface nano-architectures and their effects on the mechanical properties and corrosion behavior of Ti-based orthopedic implants[J]. Surface Coatings Technol, 2013(233):13-26.
[6]	Muñoz S, Pavón J, Rodríguez-Ortiz JA , et al. On the in-fluence of space holder in the development of porous tita-nium implants: mechanical, computational and biological evaluation[J]. Mater Charact, 2015,108:68-78. doi: 10.1016/j.matchar.2015.08.019 URL
[7]	Gu D, Shen Y . Balling phenomena during direct laser sin-tering of multi-component Cu-based metal powder[J]. J Alloys Compounds, 2007,432(1/2):163-166. doi: 10.1016/j.jallcom.2006.06.011 URL
[8]	Li X, Ma XY, Feng YF , et al. Osseointegration of chitosan coated porous titanium alloy implant by reactive oxygen species-mediated activation of the PI3K/AKT pathway under diabetic conditions[J]. Biomaterials, 2015,36:44-54. doi: 10.1016/j.biomaterials.2014.09.012 URL
[9]	Cui X, Gu Y, Li L , et al. In vitro bioactivity, cytocompati-bility, and antibiotic release profile of gentamicin sulfate-loaded borate bioactive glass/chitosan composites[J]. J Ma-ter Sci Mater Med, 2013,24(10):2391-2403. doi: 10.1007/s10856-013-4996-0 URL
[10]	戚留举, 李子夫, 张春雨 , 等. 基于选择性激光熔化制备多孔钛结构的设计和分析 [J]. 机械, 2014(5):70-74. doi: 10.3969/j.issn.1006-0316.2014.05.017 URL
	Qi LJ, Li ZF, Zhang CY , et al. Design and analysis of manu-facturing porous titanium structures based on selective laser melting[J]. Machinery, 2014(5):70-74. doi: 10.3969/j.issn.1006-0316.2014.05.017 URL
[11]	石茂林, 李洪友, 陈梦月 . 二段式钛合金种植牙不同弹性模量组件及其组合对骨界面应力分布的影响[J]. 华侨大学学报(自然科学版), 2014(4):361-366.
	Shi ML, Li HY, Chen MY . Influence of two-section tita-nium alloy dental implant components with different elastic modulus and their combinations on implant-bone interface stress distribution[J]. J Huaqiao Univ (Nat Sci), 2014(4):361-366.
[12]	Besong AA, Hailey JL, Ingham E , et al. A study of the com-bined effects of shelf aging following irradiation in air and counterface roughness on the wear of UHMWPE[J]. Bio-med Mater Eng, 1997,7(1):59-65.

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选择性激光烧结多孔钛种植体及其性能研究

Selective laser sintering and performances of porous titanium implants

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