华西口腔医学杂志

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牙周炎患者龈下菌斑与口臭相关性的研究

刘 怡1,黄海云1,章锦才2,王松灵3   

  1. 1.口腔生物医学工程教育部重点实验室,四川大学,四川 成都610041; 2.广东省口腔医院 口腔内科,广东 广州510260;3.首都医科大学北京口腔医院 涎腺治疗中心,北京100050
  • 收稿日期:2004-12-25 修回日期:2004-12-25 出版日期:2004-12-20 发布日期:2004-12-20
  • 通讯作者: 章锦才,Tel:020-84241308
  • 作者简介:刘 怡(1972-),女,四川人,主治医师,现为首都医科大学北京口腔医院博士研究生

The Study of the Relationship of Malodor and Microbial Composition of Interdental and Subgingival Plaques in Periodon- titis Patients

LIUYi1,HUANGHai-yun1,ZHANGJin-cai2,WANG Song-ling3   

  1. 1.Key.Laboratory ofOral Biomedical En- gineering Ministry ofEducation,Sichuan University,Chengdu610041,China; 2.Dept.ofOralMedicine,GuangdongProvincial Hospital ofStomatology,Guangzhou510260,China;3.Salivary Gland Disease Center,BeijingHospitalfor Stomatology,Capital University ofMedical Sciences,Beijing100050,China
  • Received:2004-12-25 Revised:2004-12-25 Online:2004-12-20 Published:2004-12-20

摘要:

目的 了解口臭与牙周炎患者龈下菌斑组成的关系。方法 用H2S测定仪Halimeter与专业医师鼻测两种方法对210例牙周炎患者进行口气测定,选取符合纳入标准的20例口臭患者作为实验组,另随机选取10例无口臭的牙周炎患者作为对照组。收集两组患者邻面间隙的颈缘菌斑和龈下菌斑行刚果红负性染色及厌氧培养,对培养出的主要细菌作分离及鉴定。所有实验结果采用SPSS 10.0进行统计学分析。结果 ①两组患者的菌斑指数、牙龈出血指数、牙周袋深度无显著性差异。②两组患者邻面间隙的颈缘菌斑和龈下菌斑中螺旋体所占百分比均有显著性差异。③两组患者邻面间隙的颈缘菌斑及龈下菌斑中的细菌检出量均无显著性差异。④两组患者邻面间隙的颈缘菌斑及龈下菌斑中主要细菌的检出率均无显著差异;与口臭相关的细菌(牙龈卟啉单胞菌、韦荣菌和二氧化碳噬纤维菌)的构成比在邻面间隙的颈缘菌斑中为实验组显著高于对照组,而在龈下菌斑中两组无显著差异。结论 并非所有的牙周炎患者均有口臭;牙周炎患者邻面间隙的颈缘菌斑中细菌的构成比与口臭的关系更密切。

关键词: 牙周炎, 口臭, 菌斑, 细菌, 厌氧培养

Abstract:

Objective The study is to investigate the microbial composition of interdental and subgingival plaques of periodon- titis patientswith orwithoutmalodor, to explore the relationships between periodontitis and oral malodor.Methods 20 patients of periodontitis with malodor were chosen from 210 patients of periodontitis, and the clinical parameter of plaque index (PLI), gingi- val bleeding index (GBI) and probing depth (PD) were measured and compared with the control groupwhich had periodontal dis- ease without malodor. During the experiment, the interdental and subgingival microbial samples in both groups were collected and sent to anaerobic culture for 48 hrs, then the total CFU/ml of each sample were counted, and each type of bacteria was separated and identified. All of the data were analyzed by using the statistical software SPSS 10.0.Results (1)There were no satistical differences on PLI, GBI, PD between experimental group and control group. (2) The percents of leptospira in both interdental and subgingival plaques of test groupwere significantly higherthan that of the control group (P<0.01). (3) Eitherthe interdental or in subgingival plaques, the count results of CFU/mlwere similar in both groups (P>0.05). (4) The proportions of malodor pro- ducing anaerobic bacteria in interdental gingival plaque, such asP.gingivalisandVeillonella, were singnificantly different be- tween test group and control group.Conclusion The proportions of VSC′s producing anaerobic bacteria in interdental gingival plaque may be play the significant roles in oral malodor. Further studies should be taken to elucidate the relationship between mal- odor and periodontitis.

Key words: periodontitis, oral malodor, plaque, bacteria, anaerobic culture