关键词: 增殖细胞核抗原, bcl-2, 免疫组织化学, 瓷熔附金属全冠, 变色

Abstract:

Objective　To studythe expression of PCNA、bcl-2 protein in gray and normal gingival tissue, and to investigate the biological effect of procelain-fused-to-metals(PFM).Methods　Gray gingival tissue animal model was established by PFM pros- thesis and immunohistochemistry S-P method was used to detect the PCNA、bcl-2 protein expression in gingival tissue after 3 months, 6 months in PFM groups and control group.Results　The expression of PCNAhad significant difference between the 3th month group and the control group(P<0.05)|no significant difference was found between the 6th month group and the control group(P>0.05). The expression of bcl-2 had no significant difference between the 6th month group and the control group(P> 0.05).Conclusion　There were a correlation between the expression of PCNA and inflammation of the gray gingival tissue;The expression of PCNA and bcl-2 protein does not indicate that gray gingival tissue had dysplasia change.

Key words: proliferating cell nuclear antigen, bcl-2, immunohistochemistry, porcelain-fused-to-metals crown, discol- oration