关键词: 凋亡指数, 增殖指数, 增殖细胞核抗原, Bcl-2阳性细胞指数, 舌癌, 化疗

Abstract:

Objective:The purpose of this study was to investigate effects of chemotherapy on tongue squamous cell carcinoma by detecting the apoptosis, proliferation and expression of Bcl-2.Methods: A total of 60 samples were studied, including tongue squamous cell carcinomas with chemotherapy (10 cases), tongue squamous cell carcinomas (30 cases), tongue squamous cell papillomas (20 cases) . The samples were investigated using immunohistochemical stain and in situ detection technique. Results: The apoptosis index (AI) was significantly lower in the group of squamous cell carcinomas compared with the squamous cell papillomas, but the proliferation index (PI) in the former group was significantly higher than the latter( P < 0.05). Compared with the group of squamous cell carcinomas, the apoptosis index increased, but proliferation index decreased in the group of chemotherapy ( P < 0.05). Bcl-2 positive cell index (BI) in the group of squamous carcinomas significantly decreased after chemotherapy (P < 0.05). hi the group of squamous cell carcinomas with chemotherapy, AI was negatively correlated with PI (r= -0.739) and, BI was negatively related with AI (r= -0.787) . Conclusion: The mechanism of chemotherapy is associated with inhibition of proliferation and induction of apoptosis, which may be determined by decreasing the expression of Bcl-2. It indicates that the pre-opera-tive chemotherapy is important for clinical prognosis of patients with tongue squamous carcinomas.

Key words: apoptosis index, proliferation index, Bcl-2 positive cell index, tongue squamous cell carcinoma chemotherapy