关键词: 溴脱氧尿嘧啶核苷, 舌癌, 肿瘤, 体外标记

Abstract:

Objective:The aim of this study is to evaluate of Bromodeoxyuridine (BrdU) label in the proliferation of the tongue cancer. Methods:The authors used 80LgPml BrdUoriododeoxyuridine (IdU) to label three cases of fresh tissue of tongue cancer and four bottles of Tca8113 cells in logarithmic growth period in vitro. Theywere fixed and embedded in the routine procedure, and then in serial sections. These specimenswere labeled with BrdUpolyclonal antibody (1B200), and PCNAmonoclonal antibody (1B100), stained using immunohistochemical ABC method. The authors observed the shapes and sizes of those positive cells, counted their label indexes (LI) of BrdU- or IdU-positive cells and PCNA- positive cells respectively. Four bottles of Tca8113 cells were cul- tured for 1~4 days. One bottle of the cells was every day used in the experimental study about comparing the cells labeled BrdU with those of the S phase of FCMin same cultural time.Results:The positive cells generally owned a larger round nucleus, some- times in company with sister cells. There was no difference of positive cells in these two kinds of labeling methods between the in- corporation of BrdUor IdUand the expression of proliferating cell nuclear antigen (P>0.05). The cells of S phase with FCM method were much more than thatmethod with BrdUincorporation (P<0.001).Conclusion: The method of BrdUlabeling could be used in tumor cellular proliferation studies.

Key words: bromodeoxyuridine, tongue cancer, tumor label in vitro