芦荟大黄素通过血小板凝血酶蛋白-1-PI3k-Akt通路抑制瘢痕组织纤维化的研究

doi:10.7518/hxkq.2025.2024422

华西口腔医学杂志 ›› 2025, Vol. 43 ›› Issue (5): 636-647.doi: 10.7518/hxkq.2025.2024422

• 基础研究 • 上一篇

芦荟大黄素通过血小板凝血酶蛋白-1-PI3k-Akt通路抑制瘢痕组织纤维化的研究

耿红宝¹(), 张星懿², 周思玮³, 李娜⁴, 刘佳⁴, 苑学微⁴, 宁春柳², 张旭东², 黄威²()

^1.石家庄市妇幼保健院口腔科，石家庄 050000
^2.河北医科大学口腔医学院·口腔医院河北省口腔医学重点实验室河北省口腔疾病临床医学研究中心，石家庄 050017
^3.石家庄市第四医院口腔科，石家庄 050011
^4.石家庄市第二医院口腔科，石家庄 050051

收稿日期:2024-11-17 修回日期:2025-05-08 出版日期:2025-10-01 发布日期:2025-10-21
通讯作者: 黄威 E-mail:861181470@qq.com;huangwei@hebmu.edu.cn
作者简介:耿红宝，主治医师，硕士，E-mail：861181470@qq.com
基金资助:
河北省医学科学研究课题计划(20240510)

Aloe-emodin inhibits scar tissue fibrosis through thrombospondin-1-PI3k-Akt pathway

Geng Hongbao¹(), Zhang Xingyi², Zhou Siwei³, Li Na⁴, Liu Jia⁴, Yuan Xuewei⁴, Ning Chunliu², Zhang Xudong², Huang Wei²()

^1.Dept. of Stomatology, Women and Children's Health Hospital in Shijiazhuang, Shijiazhuang 050000, China
^2.Hebei Key Laboratory of Stomatology, Hebei Clinical Research Center for Oral Disease, School and Hospital of Stomatology, Hebei Medical University, Shijiazhuang 050017, China
^3.Dept. of Stomatology, Shijiazhuang Fourth Hospital, Shijiazhuang 050011, China
^4.Dept. of Stomatology, Shijiazhuang Second Hospital, Shijiazhuang 050051, China.

Received:2024-11-17 Revised:2025-05-08 Online:2025-10-01 Published:2025-10-21
Contact: Huang Wei E-mail:861181470@qq.com;huangwei@hebmu.edu.cn
Supported by:
Medical Science Research Project in Hebei Province(20240510)

摘要/Abstract

摘要：

目的探讨芦荟大黄素通过血小板凝血酶蛋白-1（THBS1）—磷脂酰肌醇3-激酶（PI3K）—蛋白激酶B（Akt）抑制瘢痕组织纤维化的机制。方法对人腭裂术后瘢痕组织来源成纤维细胞进行培养，采用不同浓度（10、20、30、40、50 μmol/L）芦荟大黄素作用于该细胞并检测其细胞活性，同时对瘢痕组织及细胞进行转录组测序，采用生物信息学方法挖掘瘢痕组织纤维化的潜在靶点及信号通路。结果芦荟大黄素对成纤维细胞增殖有抑制作用，其中40 μmol/L浓度组最明显；瘢痕组织及细胞测序结果提示，差异基因在细胞外基质与受体相互作用通路中显著富集，且组织与细胞拥有共同差异基因THBS1；过表达分析结果提示，差异基因THBS1在PI3K-Akt信号通路中显著富集。结论芦荟大黄素可能通过下调THBS1，抑制PI3K-Akt通路，从而降低术后腭部瘢痕组织来源的成纤维细胞增殖活性。

关键词: 芦荟大黄素, 血小板凝血酶蛋白-1, PI3K-Akt信号通路, 纤维化, 腭部瘢痕

Abstract:

Objective To propose a hypothesis that aloe-emodin may inhibit scar tissue fibrosis through thrombospondin-1(THBS1)-PI3K-Akt pathway. Methods By cultivating fibroblasts derived from scar tissue after cleft palate surgery in humans, aloe emodin of different concentrations (10, 20, 30, 40 and 50 μmol/L) was added to the cells which activity was detected. At the same time, transcriptome sequencing was performed on scar tissue and cells, and bioinformatics methods were used to explore potential targets and signaling pathways of scar tissue fibrosis. Results Aloe-emodin had a concentration dependent inhibitory effect on fibroblast proliferation,with the 40 μmol/L concentration group showing the most significant effect. The results of tissue and cell sequencing indicated that differentially expressed genes were significantly enriched in extracellular matrix-receptor interaction pathway, and shared a common differential gene which was THBS1. The ORA analysis results indicated that differentially expressed genes, including THBS1, were significantly enriched in the PI3K-Akt signaling pathway. Conclusion Aloe emodin may inhibit the PI3K-Akt pathway by downregulating THBS1, thereby reducing the proliferation activity of fibroblasts derived from postoperative palatal scar tissue.

Key words: aloe-emodin, thrombospondin-1, PI3K-Akt signaling pathway, fibrosis, palatal scar

中图分类号:

R782.2

耿红宝, 张星懿, 周思玮, 李娜, 刘佳, 苑学微, 宁春柳, 张旭东, 黄威. 芦荟大黄素通过血小板凝血酶蛋白-1-PI3k-Akt通路抑制瘢痕组织纤维化的研究[J]. 华西口腔医学杂志, 2025, 43(5): 636-647.

Geng Hongbao, Zhang Xingyi, Zhou Siwei, Li Na, Liu Jia, Yuan Xuewei, Ning Chunliu, Zhang Xudong, Huang Wei. Aloe-emodin inhibits scar tissue fibrosis through thrombospondin-1-PI3k-Akt pathway[J]. West China Journal of Stomatology, 2025, 43(5): 636-647.

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芦荟大黄素通过血小板凝血酶蛋白-1-PI3k-Akt通路抑制瘢痕组织纤维化的研究

Aloe-emodin inhibits scar tissue fibrosis through thrombospondin-1-PI3k-Akt pathway

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