关键词: 慢性间歇低氧, 阻塞性睡眠呼吸暂停低通气综合征, 牙周炎, 白细胞介素-18, 低氧诱导因子-1α

Abstract:

Objective This study evaluates the expression of interleukin-18 (IL-18) and hypoxia-inducible factor (HIF)-1αin rat periodontitis model exposed to normoxia and chronic intermittent hypoxia (CIH) environments. The possible correlation between periodontitis and obstructive sleep apnea-hypopnea syndrome (OSAHS) was also investigated. Methods Thirty-two Sprague–Dawley (SD) rats were randomly assigned into four groups: normoxia control, normoxia periodontitis, hypoxia control, and hypoxia periodontitis groups. The periodontitis models were established by ligating the bilateral maxillary second molars and employing high-carbohydrate diets. Rats in hypoxia control and hypoxia periodontitis groups were exposed to CIH treatment mimicking a moderately severe OSAHS condition. All animals were sacrificed after eight weeks, and the clinical periodontal indexes were detected. The levels of IL-18 and HIF-1α in serum and gingival tissues were determined using enzyme-linked immunosorbent assay (ELISA). The correlation between attachment loss (AL) and the levels of IL-18 and HIF-1α in hypoxia periodontitis group was evaluated. Results The levels of IL-18 and HIF-1α in hypoxia periodontitis group were significantly higher than that in normoxia periodontitis and hypoxia control groups (P<0.05). Furthermore, the levels of IL-18 and HIF-1α in serum (r=0.792, r=0.753, P<0.05) and gingival tissues (r=0.817, r=0.779, P<0.05) were positively correlated with AL. Conclusion CIH could aggravate the destruction of periodontal tissues, which is correlated with IL-18 and HIF-1α levels.

Key words: chronic intermittent hypoxia, obstructive sleep apnea-hypopnea syndrome, periodontitis, interleukin-18, hypoxia-inducible factor-1α