关键词: 咬合创伤, 细胞间通讯, 缝隙连接, 焦点粘连, 紧密连接, 黏着连接

Abstract:

Objective To study the intercellular communication of alveolar bone during traumatic occlusion at early stage in rats. Methods The occlusal surface of the upper left first molar of rat was raised by placing a stainless steel wire to induce occlusal trauma in the lower left first molar. After 24 hours, the alveolar bone tissues of the lower jaws first molars at the both sides were taken out under anesthesia. The various 27 000 genes were identified with genomewide microarray, and further were investigated with reverse transcription-polymerase chain reaction（RT-PCR） and Pathway analysis. Results Total 586 gene were found to be changed, 106 different signal pathways got involved with Pathway analysis, including cell adhesion molecules（CAMS）, adhesions junction, gap junction, focal adhesion and tight junction, and the cytokines associated with bone metabolism in above 5 signal pathways were all down-regulated. Conclusion At the early phase of the occlusal trauma, intercellular communication in rat’s alveolar bone were inhibited.

Key words: traumatic occlusion, intercellular communication, gap junction, focal adhesion, tight junction, adhesions junction