恒压式葡萄糖定量法评价4种根管封闭剂的封闭性能

doi:10.3969/j.issn.1000-1182.2012.05.006

华西口腔医学杂志 ›› 2012, Vol. 30 ›› Issue (5): 474-477.doi: 10.3969/j.issn.1000-1182.2012.05.006

恒压式葡萄糖定量法评价4种根管封闭剂的封闭性能

穆云静1，2 屈铁军2 程小刚2 王小勤2 赵明1 汪雨娜3 韩冰2 余擎2

1.解放军第309医院旃坛寺门诊部口腔科，北京100034； 2.第四军医大学口腔医院牙体牙髓科，西安710032；3.解放军第515医院口腔科，江阴214431

收稿日期:2012-01-20 修回日期:2012-08-07 出版日期:2012-10-01 发布日期:2012-10-01
通讯作者: 穆云静（1978—），女，陕西人，主治医师，硕士
作者简介:穆云静（1978—），女，陕西人，主治医师，硕士
基金资助:
无

A quantitative glucose method by constant air pressure for evaluating the sealing ability of four root canal sealers

Mu Yunjing1，2, Qu Tiejun2, Cheng Xiaogang2, Wang Xiaoqin2, Zhao Ming1, Wang Yuna3, Han Bing2, Yu Qing2.

1. Dept. of Stomatology, Zhantansi Clinic, 309 Hospital of PLA, Beijing 100034, China; 2. Dept. of Operative Dentistry and Endodontics, School of Stomatology, The Fourth Military Medical University, Xi’an 710032, China; 3. Dept. of Stomatology, 515 Hospital of PLA, Jiangyin 214431, China

Received:2012-01-20 Revised:2012-08-07 Online:2012-10-01 Published:2012-10-01

摘要/Abstract

摘要：

目的建立恒压式葡萄糖定量微渗漏模型，并以此评价AH Plus、Roeko Seal、Cortisomol、RC Sealer 4种根管封闭剂的封闭性能。方法选取56颗单直根管的上前牙，随机分为4个实验组：AH Plus组（A组）、RoekoSeal组（B组）、Cortisomol组（C组）、RC Sealer组（D组），每组各12颗；另设阳性对照组和阴性对照组，每组各4颗。常规根管预备后，实验组用冷牙胶侧向加压充填技术配合各根管封闭剂充填根管，对照组只使用牙胶尖充填。通过恒压式葡萄糖定量微渗漏模型，用葡萄糖氧化酶-过氧化物酶法（GOD-POD）检测第1、3、5、7、10、15、20、25、30天从冠方向根方漏出的葡萄糖的量。结果第1、3、5天时，A、B两组间及C、D两组间葡萄糖微渗漏值差异无统计学意义（P>0.05）；第7天起实验各组微渗漏值逐渐增大，且实验各组间差异均有统计学意义（P<0.05）。结论恒压式葡萄糖微渗漏模型建模简单、灵敏度高、操作性强。聚二甲基硅氧烷类根管封闭剂（Roeko Seal）封闭根管的能力较强，树脂类（AH Plus）居中，氧化锌类（Cortisomol、RC Sealer）较弱。

关键词: 根管封闭剂, 微渗漏, 葡萄糖氧化酶-过氧化物酶法

Abstract:

Objective To introduce a modified model for quantitative testing of glucose microleakage by constant air pressure and evaluate the sealing ability of four root canal sealers including AH Plus, Roeko Seal, Cortisomol and RC Sealer. Methods Fifty-six straight maxillary anterior teeth were randomly divided into four experimental groups with 12 samples in each group, group A: AH Plus, group B: Roeko Seal, group C: Cortisomol, group D: RC Sealer, and positive and negative control group with 4 samples in each. After regular root preparation, samples in experimental groups were obturated by cold gutta-percha lateral compaction technique with sealers according to group description. Control groups were obturated with cold gutta-percha only. Through the model above, the value of glucose microleakage
from crown to root was measured at 1, 3, 5, 7, 10, 15, 20, 25, 30 days with the glucose oxidase-peroxidase method （GOD-POD）. Results At day 1, 3, 5, no significant difference of microleakage was found between group A and B （P>0.05）. The same result was also seen between group C and D. From 7 days, microleakage value in each experimental group gradually increased, and statistical difference was found between groups（P<0.05）. Conclusion This new
model using constant air pressure is easy to establish, sensitive and practical. Among all the sealers evaluated, polydimethylsiloxane based root canal sealer, Roeko Seal has the greatest sealing ability, while epoxy-amine resin based AH Plus is second and Zinc Oxide based Cortisomol and RC Sealer have the least sealing ability.

Key words: root canal sealer, microleakage, glucose oxidase-peroxidase method

穆云静1，2 屈铁军2 程小刚2 王小勤2 赵明1 汪雨娜3 韩冰2 余擎2. 恒压式葡萄糖定量法评价4种根管封闭剂的封闭性能[J]. 华西口腔医学杂志, 2012, 30(5): 474-477.

Mu Yunjing1，2, Qu Tiejun2, Cheng Xiaogang2, Wang Xiaoqin2, Zhao Ming1, Wang Yuna3, Han Bing2, Yu Qing2.. A quantitative glucose method by constant air pressure for evaluating the sealing ability of four root canal sealers[J]. West China Journal of Stomatology, 2012, 30(5): 474-477.

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恒压式葡萄糖定量法评价4种根管封闭剂的封闭性能

A quantitative glucose method by constant air pressure for evaluating the sealing ability of four root canal sealers

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