West China Journal of Stomatology ›› 2021, Vol. 39 ›› Issue (5): 518-523.doi: 10.7518/hxkq.2021.05.004

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Effect of peroxisome proliferator-activated receptor-γ coactivator-1α on liver injury induced by periodontitis in rats

Xia Boyuan1(), Li Yan1, Ding Xu1, Li Xin1, Liu Xinchan2, Yu Weixian2,3()   

  1. 1.Dept. of Periodontics, Hospital of Stomatology, Jilin University, Changchun 130021, China
    2.Dept. of Geriatric Stomatology, Hospital of Stomatology, Jilin University, Changchun 130021, China
    3.Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Changchun 130021, China
  • Received:2020-08-12 Revised:2021-07-08 Online:2021-10-01 Published:2021-10-11
  • Contact: Yu Weixian E-mail:1490431774@qq.com;yu-wei-xian@163.com
  • Supported by:
    The Jilin Provincial Science and Technology Project of Finance Department(JCSZ2019378-1);The Jilin Provincial Science and Technology Project(20190201058JC);The Jilin Provincial Science and Technology International Cooperation Project(20180414053GH);Correspondence: Yu Weixian, E-mail: yu-wei-xian@163.com

Abstract: Objective

To investigate the effect of peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) on liver injury induced by periodontitis in rats.

Methods

Twenty-four male Wistar rats were randomly divided into two groups: control group and periodontitis group, twelve per group. In periodontitis group, the periodontitis models were established for the maxillary first molars in rats by means of “wire ligation+vaccinationwith Porphyromonas gingivalis”, the control group was inoculated with the equal volume of 2% sodium carboxymethyl cellulose in the same position, for 6 weeks. The probing depth, tooth mobility and sulcus bleeding index were detected. Hematoxylin-eosin (HE) staining was used to observe the pathological changes of liver tissues in rats. The quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC) were used to detect the gene and protein expression levels of PGC-1α, nuclear factor erythroid 2-related factor 2 (Nrf2) and mitochondrial transcription factor A (TFAM) in liver tissues of rats.

Results

The probing depth, tooth mobility and sulcus bleeding index in periodontitis group were significantly higher than that in control group. HE staining showed in periodontitis group, hepatic cords ranged disorderly and there were vacuoles in cells and inflammatory cells infiltrated in liver tissues of rats, and there was no obvious abnormality in control group. The qRT-PCR results showed that the mRNA expression levels of Pgc-1α, Nrf2 and Tfam in liver tissues of rats in periodontitis group were lower obviously than that in control group. IHC results showed that the protein expression level of PGC-1α in liver tissues of rats in periodontitis group was decreased significantly than that in control group.

Conclusion

PGC-1α may be involved in the process of periodontitis-induced liver injury in rats.

Key words: periodontitis, liver injury, peroxisome proliferator-activated receptor-γ coactivator-1α

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