Salivary microbial communities associated with severe early childhood caries

doi:10.7518/hxkq.2018.02.007

Abstract

Abstract:

Objective To compare the salivary microbial profiles of healthy subjects and those with severe early childhood caries (S-ECC) by using high-throughput sequencing. Methods Salivary samples were obtained from children with S-ECC (group C, n=24) and healthy children (group H,n=24). Total metagenomic DNA was extracted, and DNA amplicons of the V1-V3 hypervariable region of the 16S rRNA gene were generated and subjected to 454 sequencing. The characteristics of oral microbial communities from the two groups were compared based on microbial diversity and taxonomy assignment. Results First, the microbial richness was significantly higher in group C than group H (P<0.05). Second, the microbial com-munity structure was significantly different for the groups H and C (P<0.01). In addition, caries microbiota was significantly conserved in group C (P<0.001). High expression of suspected cariogenic microorganisms in group C (P<0.1) and health related microorganisms in group H (P<0.1) were identified. Finally, models of caries risk assessment were proposed to distin-guish caries from healthy subjects with over 70% accuracy.Conclusion Salivary microbiota and certain taxa, such as caries-associated taxa (Prevotella), may be useful to screen/assess the children’s risk of developing caries.

Key words: early childhood caries, oral microbiota, saliva, metagenomic

CLC Number:

R788

Tongzheng Sun, Fei Teng, Songbo Jia, Yongping Tang, Ming Jiang, Shi Huang, Xiao Yuan, Xiaolan Li, Fang Yang. Salivary microbial communities associated with severe early childhood caries[J]. West China Journal of Stomatology, 2018, 36(2): 150-155.

Figures/Tables 5

References 18

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