Effects of geranylgeranyltransferaseⅠsilencing on the proliferation of tongue squamous cancer cells

doi:10.7518/hxkq.2017.04.006

Abstract

Abstract:

Objective This study aims to investigate the effect of geranylgeranyltransferaseⅠ (GGTase-Ⅰ) on the proliferation and growth of tongue squamous cancer cells. Methods Three small interfering RNAs (siRNAs) were designed on the basis of the GGTase-Ⅰ sequence in GeneBank. These siRNAs were then transfected into tongue squamous cancer cells Cal-27. The mRNA and protein expression of GGTase-Ⅰ and RhoA were examined by real-time quantitative polymerase chain reaction and Western blotting, respectively. The expression of Cyclin D1 and p21 were examined by Western blotting. The proliferation and growth ability were analyzed by cell counting kit-8 assay and flow cytometry. Results The mRNA and protein expression of GGTase-Ⅰ in Cal-27 was reduced significantly after the GGTase-Ⅰ siRNAs were transfected (P<0.05). No significant difference in RhoA mRNA and protein expression was detected (P>0.05). Cyclin D1 expression decreased, whereas p21 expression increased significantly. The cell cycle was altered, and the growth-proliferative activity was inhibited (P<0.05). Conclusion GGTase-Ⅰ siRNA can inhibit the expression of GGTase-Ⅰ and the proliferative activity of tongue squamous cancer cells. GGTase-Ⅰ may be a potential target for gene therapy in tongue squamous cell cancer.

Key words: geranylgeranyltransferaseⅠ, RhoA, tongue squamous cancer, proliferation, Cyclin D1, p21

CLC Number:

R739.8

Ying Wang, Qimin Wang, Jinghua Li, Jinhong Han, Lili Wang, Chen Chao, Jianhua Zhou, Lei Tong, Xufei Lu, Yuan Zhou, Yixiang Liao, Zongxuan He, Ning Li, Lei Cao, Wenjun Liu, Zhenggang Chen. Effects of geranylgeranyltransferaseⅠsilencing on the proliferation of tongue squamous cancer cells[J]. West China Journal of Stomatology, 2017, 35(4): 373-378.

Figures/Tables 6

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siRNA	产品编号	产品名称	靶序列
GGTase-ⅠsiRNA 1	siG000005229A	si-h-PGGT1B_001	GGAACAGCTCATCCTTATG
GGTase-ⅠsiRNA 2	siG000005229B	si-h-PGGT1B_002	CGACTTAAGCCGAGTAAAT
GGTase-ⅠsiRNA 3	siG000005229C	si-h-PGGT1B_003	GACTTCTGAACGCCTTCTA