慢性牙周炎患者唾液蛋白酶谱分析

doi:10.7518/hxkq.2017.01.005

华西口腔医学杂志 ›› 2017, Vol. 35 ›› Issue (1): 37-42.doi: 10.7518/hxkq.2017.01.005

慢性牙周炎患者唾液蛋白酶谱分析

李倩(), 周学东, 樊亚平, 杨腾宇, 吴松涛, 余宇, 陈娇, 张平, 冯云()

口腔疾病研究国家重点实验室华西口腔医院（四川大学）,成都 610041

收稿日期:2016-02-16 修回日期:2016-06-10 出版日期:2017-02-01 发布日期:2017-02-01
作者简介:
李倩,硕士,E-mail：996462491@qq.com
基金资助:
国家自然科学基金（81372892）;四川省科技厅平台建设项目（JCPT 2011-9）

Analysis of salivary protease spectrum in chronic periodontitis

Qian Li(), Xuedong Zhou, Yaping Fan, Tengyu Yang, Songtao Wu, Yu Yu, Jiao Chen, Ping Zhang, Yun. Feng()

State Key Laboratory of Oral Diseases, West China Hospital of Sto-matology, Sichuan University, Chengdu 610041, China

Received:2016-02-16 Revised:2016-06-10 Online:2017-02-01 Published:2017-02-01
Supported by:
The National Natural Science Foundation of China (81372892);Sichuan Province Science and Technology Innovation Team Program (JCPT 2011-9)

摘要/Abstract

摘要：

目的比较慢性牙周炎患者和牙周健康者的唾液蛋白谱差异,以期为慢性牙周炎的诊断和治疗监测提供依据。方法收集慢性牙周炎患者和牙周健康者刺激性唾液,采用蛋白芯片技术,对慢性牙周炎患者和正常人的唾液蛋白酶谱进行分析。结果慢性牙周炎患者和牙周健康者唾液蛋白酶的表达具有统计学差异。其中,慢性牙周炎患者唾液中去整合素金属蛋白酶（ADAM）8,基质金属蛋白酶（MMP）-8、-12,脑啡肽酶/CD10,尿激酶纤维蛋白溶酶原激活剂/尿激酶的表达高于牙周健康者（P<0.01）;ADAM9,含凝血酶敏感素基序的去整合素金属蛋白酶（ADAMTS）1、13,组织蛋白酶B、E、L、V、X/Z/P,激肽释放酶6、7、11、13,MMP-9,蛋白酶3、早老素1和前蛋白转化酶9的表达低于牙周健康者（P<0.05）。结论慢性牙周炎患者的唾液蛋白酶谱与牙周健康者具有显著差异,唾液蛋白酶谱分析将有望成为慢性牙周炎临床诊断和治疗监测的实验检查手段。

关键词: 唾液, 慢性牙周炎, 蛋白酶谱

Abstract:

Objective This study aimed to investigate the difference in salivary protease expression in patients with chro-nic periodontitis and normal individuals. Methods The stimulating saliva in patients with chronic periodontitis and normal individuals were collected. Protein chip technology was adapted to analyze salivary protease spectrum. Results Among the 34 proteases in the chip, disintegrin and metalloproteinase (ADAM)8, matrix metalloproteinase (MMP)-8, MMP-12, neprilysin/CD10, and uridylyl phosphate adenosine/urokinase showed a significantly increased concentration in the saliva of chronic periodontitis patients compared with those in the saliva of normal individuals (P<0.01). By contrast, the concentrations of ADAM9, a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)1, ADAMTS13, cathepsin B, E, L, V, X/Z/P, kallikrein 6, 7, 11, 13,MMP-9, proteinase 3, presenilin-1, and proprotein convertase 9 sharply decreased (P<0.05). Conclusion The results demonstrated that protease spectrum in the saliva of chronic periodontitis patients and normal indi-viduals significantly differed. Analysis of salivary protease spectrum is a potential clinical method to examine, diagnose, and monitor chronic periodontitis.

Key words: saliva, chronic periodontitis, protease spectrum

中图分类号:

R781.4

李倩, 周学东, 樊亚平, 杨腾宇, 吴松涛, 余宇, 陈娇, 张平, 冯云. 慢性牙周炎患者唾液蛋白酶谱分析[J]. 华西口腔医学杂志, 2017, 35(1): 37-42.

Qian Li, Xuedong Zhou, Yaping Fan, Tengyu Yang, Songtao Wu, Yu Yu, Jiao Chen, Ping Zhang, Yun. Feng. Analysis of salivary protease spectrum in chronic periodontitis[J]. West China Journal of Stomatology, 2017, 35(1): 37-42.

图/表 2

参考文献 21

[1]	Van Nieuw Amerongen A, Bolscher JG, Veerman EC. Sali-vary proteins: protective and diagnostic value in cariology[J]. Caries Res, 2004, 38(3):247-253.
[2]	Chiappin S, Antonelli G, Gatti R, et al.Saliva specimen: a new laboratory tool for diagnostic and basic investigation[J]. Clin Chim Acta, 2007, 383(1/2):30-40.
[3]	Helmerhorst EJ, Oppenheim FG.Saliva: a dynamic proteome[J]. J Dent Res, 2007, 86(8):680-693.
[4]	Chaudhry AS.Comparing two commercial enzymes to esti-mate in vitro proteolysis of purified or semi-purified pro-teins[J]. J Anim Physiol Anim Nutr (Berl), 2005, 89(11/12):403-412.
[5]	Boudida Y, Gagaoua M, Becila S, et al.Serine protease in-hibitors as good predictors of meat tenderness: which are they and what are their functions[J]. Crit Rev Food Sci Nutr, 2016, 56(6):957-972.
[6]	Lima DP, Diniz DG, Moimaz SA, et al.Saliva: reflection of the body[J]. Int J Infect Dis, 2010, 14(3):e184-e188.
[7]	Armitage GC.Development of a classification system for periodontal diseases and conditions[J]. Ann Periodontol, 1999, 4(1):1-6.
[8]	Schipper RG, Silletti E, Vingerhoeds MH.Saliva as research material: biochemical, physicochemical and practical aspects[J]. Arch Oral Biol, 2007, 52(12):1114-1135.
[9]	Smith GA, Riedford K.Epidemiology of early childhood caries: clinical application[J]. J Pediatr Nurs, 2013, 28(4): 369-373.
[10]	Ng MW, Ramos-Gomez F, Lieberman M, et al.Disease management of early childhood caries: ECC collaborative project[J]. Int J Dent, 2014, 2014:327801.
[11]	Ng MW, Ramos-Gomez F.Disease prevention and mana-gement of early childhood caries[J]. J Mass Dent Soc, 2012, 61(3):28-32.
[12]	Guarino C, Legowska M, Epinette C, et al.New selective peptidyl di(chlorophenyl) phosphonate esters for visualizing and blocking neutrophil proteinase 3 in human diseases[J]. J Biol Chem, 2014, 289(46):31777-31791.
[13]	Rao NV, Wehner NG, Marshall BC, et al.Characterization of proteinase-3 (PR-3), a neutrophil serine proteinase. Struc-tural and functional properties[J]. J Biol Chem, 1991, 266(15):9540-9548.
[14]	Yang TY, Zhou WJ, Du Y, et al.Role of saliva proteinase 3 in dental caries[J]. Int J Oral Sci, 2015, 7(3):174-178.
[15]	蔡静莉, 杨凌峰, 王蓉, 等. ADAM家族研究进展[J]. 国外医学分子生物学分册, 2000, 22(4):197-201.
	Cai JL, Yang LF, Wang R, et al.Research progress of ADAM family[J]. J Med Mol Biol, 2000, 22(4):197-201.
[16]	郭丽丽, 郭胜利, 郭炜. ADAMTS家族在肿瘤中的研究进展[J]. 医学综述, 2015, 21(11):1975-1979.
	Guo LL, Guo SL, Guo W.Research progress of a disintegrin and metalloproteinase with thrombospondin family in tumor[J]. Med Recapitul, 2015, 21(11):1975-1979.
[17]	Inuzuka K, Ogata Y, Nagase H, et al.Significance of coex-pression of urokinase-type plasminogen activator, and matrix metalloproteinase 3 (stromelysin) and 9 (gelatinase B) in colorectal carcinoma[J]. J Surg Res, 2000, 93(2):211-218.
[18]	华小川, 赖文莉. 脑啡肽及其与牙疼痛关系的研究进展[J]. 国际口腔医学杂志, 2007, 34(3):197-199.
	Hua XC, Lai WL.Development of research of enkephalin in tooth pain[J]. Int J Stomatol, 2007, 34(3):197-199.
[19]	李磊, 李岩. Kallikrein 10的调控机制及其肿瘤临床应用的价值[J]. 中国肿瘤生物治疗杂志, 2014, 21(1):109-113.
	Li L, Li Y.Regulatory mechanisms and clinical application values of kallikrein 10 gene in cancer[J]. Chin J Cancer Bio-ther, 2014, 21(1):109-113.
[20]	Pan W, Zhu P, Huizhen YU.Advances in tissue kallikrein and TIMP on vascular remodeling[J]. Adv Cardiovasc Dis, 2014, 35(4):498-503.
[21]	王光平, 李明霞, 刘建国. 激肽释放酶4与釉质发生不全[J]. 国际口腔医学杂志, 2013, 40(4):537-539.
	Wang GP, Li MX, Liu JG.Kallikrein 4 and amelogenesis imperfecta[J]. Int J Stomatol, 2013, 40(4):537-539.

慢性牙周炎患者唾液蛋白酶谱分析

Analysis of salivary protease spectrum in chronic periodontitis

RichHTML

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 2

参考文献 21

相关文章 15

编辑推荐

Metrics

本文评价

[1]	姜健红, 石兴莲, 何权敏, 高丽, 杨琨, 王太萍, 李哲臻, 刘梅. 老年慢性牙周炎患者健康素养与生活质量的相关性研究[J]. 华西口腔医学杂志, 2023, 41(6): 694-700.
[2]	张晨, 候珍珍, 宗颖睿. 慢性牙周炎与慢性阻塞性肺疾病的潜在共同分子机制及其转录因子初探[J]. 华西口腔医学杂志, 2023, 41(5): 533-540.
[3]	侯亚丽, 李荷香, 宋鹏, 杨艳霄, 郝亚丽, 刘慧娟. 肿瘤间质成纤维细胞对体外培养唾液腺多形性腺瘤细胞生物学行为的影响[J]. 华西口腔医学杂志, 2023, 41(2): 149-156.
[4]	奚悦, 马玉婷, 姚志刚, 覃业军, 赵苗青. Warthin瘤合并T淋巴母细胞淋巴瘤1例[J]. 华西口腔医学杂志, 2022, 40(6): 727-730.
[5]	戴振宁, 郑蔚晗, 利时雨. 核因子κB受体活化因子配体和肿瘤坏死因子α经炎性牙周膜干细胞外泌体促进破骨细胞分化[J]. 华西口腔医学杂志, 2022, 40(4): 377-385.
[6]	陈娇, 吕蝶, 陈红利, 张平. 蛋白激酶D抑制剂CRT0066101对唾液腺腺样囊性癌细胞迁移能力的影响[J]. 华西口腔医学杂志, 2022, 40(3): 320-327.
[7]	魏卓, 周昊, 费伟, 聂雄, 李明芳. SOX2基因对唾液腺腺样囊性癌细胞生物学特性的影响[J]. 华西口腔医学杂志, 2022, 40(3): 341-349.
[8]	张颖, 贾松菠, 李帆, 李姗姗, 张利娟, 谭凯璇, 卢洁, 杨芳. 低龄儿童龋病相关的唾液生化指标研究[J]. 华西口腔医学杂志, 2021, 39(3): 300-305.
[9]	李审绥, 吴沉洲, 乔翔鹤, 李春洁, 李龙江. 辐射损伤唾液腺机制及治疗的研究进展[J]. 华西口腔医学杂志, 2021, 39(1): 99-104.
[10]	付汉斌, 罗琳. HOX反义基因间RNA在唾液腺腺样囊性癌组织中的表达及对预后的影响[J]. 华西口腔医学杂志, 2020, 38(5): 509-512.
[11]	张杨珩, 李厚轩, 闫福华, 谭葆春. 牙周内窥镜辅助龈下刮治对残留牙周袋疗效的随机对照临床研究[J]. 华西口腔医学杂志, 2020, 38(5): 532-536.
[12]	常雅琴, 房方方, 秦莎莎, 董迎春, 陈斌. 中重度慢性牙周炎患者牙周洁刮治前后血清C反应蛋白水平变化的系统评价和Meta分析[J]. 华西口腔医学杂志, 2020, 38(4): 385-392.
[13]	柏银, 白远亮, 赖静, 黄姣. 克拉霉素辅助治疗慢性牙周炎疗效的Meta分析[J]. 华西口腔医学杂志, 2020, 38(3): 290-296.
[14]	王苗苗, 赵勇, 王冲, 李豪, 石学雪, 任秀云. 牙周非手术治疗对伴二型糖尿病的慢性牙周炎大鼠血清白细胞介素6表达及颈动脉血管壁的影响[J]. 华西口腔医学杂志, 2019, 37(6): 589-593.
[15]	王旭,石磊,应绚,童钰鑫,张佳喻,石旭妍,陈悦. 吸烟对慢性牙周炎牙龈微循环影响的初步研究[J]. 华西口腔医学杂志, 2019, 37(5): 485-489.