Vps4b基因敲除鼠上皮根鞘中细胞角蛋白14和增殖细胞核抗原的表达

doi:10.7518/hxkq.2021.03.005

华西口腔医学杂志 ›› 2021, Vol. 39 ›› Issue (3): 274-278.doi: 10.7518/hxkq.2021.03.005

Vps4b基因敲除鼠上皮根鞘中细胞角蛋白14和增殖细胞核抗原的表达

田晴¹(), 王莹莹², 李强¹, 陈栋¹()

^1.郑州大学第一附属医院河南省口腔医院口腔科，郑州 450052
^2.徐州市中心医院口腔科，徐州 221009

收稿日期:2020-05-26 修回日期:2020-12-19 出版日期:2021-06-01 发布日期:2021-05-26
通讯作者: 陈栋 E-mail:635448763@qq.com;fccchend@zzu.edu.cn
作者简介:田晴，硕士，E-mail：635448763@qq.com
基金资助:
河南省重点研发与推广专项（科技攻关）(22170124);河南省医学科技攻关计划(SBGJ2018038)

Expressions of cytokeratin 14 and proliferating cell nuclear antigen in the Hertwig’s epithelial root sheath of a Vps4b knockout mouse

Tian Qing¹(), Wang Yingying², Li Qiang¹, Chen Dong¹()

^1.Dept. of Stomatology, The First Affiliated Hospital of Zhengzhou University, Henan Provincial Stomatological Hospital, Zhengzhou 450052, China
^2.Dept. of Stomatology, Xuzhou Central Hospital, Xuzhou 221009, China

Received:2020-05-26 Revised:2020-12-19 Online:2021-06-01 Published:2021-05-26
Contact: Chen Dong E-mail:635448763@qq.com;fccchend@zzu.edu.cn
Supported by:
Key Research and Development Project of Henan Province(22170124);Medical Science and Technique Project of Henan Province(SBGJ2018038)

摘要/Abstract

摘要： 目的

研究Vps4b基因突变对上皮根鞘（HERS）中细胞角蛋白14（CK14）和增殖细胞核抗原（PCNA）表达的影响。

方法

取出生后（P）5、9、11、15、19 d的小鼠的双侧下颌组织，石蜡包埋后获得下颌第一磨牙组织切片，通过免疫组织化学方法比较CK14和PCNA在正常小鼠与Vps4b基因敲除小鼠HERS中的表达情况。

结果

正常小鼠P5 d开始形成HERS，PCNA在HERS细胞中强阳性表达；P9 d，HERS结构连续，PCNA在HERS细胞中阳性表达；P11 d，一小部分HERS开始断裂，PCNA在HERS细胞中弱阳性表达；P15 d，HERS继续断裂，PCNA在牙根表面HERS细胞中弱阳性表达；P19 d，牙根达到生理长度，PCNA仅在牙根末端HERS细胞中阳性表达。基因敲除小鼠与正常小鼠相比，P5 d同样形成HERS结构，然而其断裂在P9 d就开始出现，P19 d牙根表面仅存少量HERS碎片，根尖发育不成熟，PCNA表达强度下降。

结论

Vps4b基因突变可能通过改变HERS中CK14和PCNA的表达，从而导致牙根发育异常。

关键词: Vps4b基因, 上皮根鞘, 细胞角蛋白14, 增殖细胞核抗原, 基因敲除

Abstract: Objective

The effect of Vps4b gene mutation on the expressions of cytokeratin 14 (CK14) and proliferating cell nuclear antigen (PCNA) in the Hertwig's epithelial root sheath (HERS) is investigated.

Methods

The bilateral mandibular tissues of mouse on postnatal days 5, 9, 11, 15, and 19 were removed. The mandibular first molar tissue sections were obtained after paraffin embedding. The CK14 and PCNA expressions in the epithelial root sheath of the normal mouse and Vps4b knockout mouse were compared through immunohistochemistry.

Results

On postnatal day 5, the normal mouse began to form HERS and had a strong positive PCNA expression in the HERS cells; on postnatal day 9, the HERS structure was continuous, and PCNA was positive in the HERS cells; on postnatal day 11, a small portion of HERS began to break, and PCNA was weakly positive in the HERS cells; on postnatal day 15, HERS continued to fracture; PCNA was weakly and positively expressed in the HERS cells on the root surface; on postnatal day 19, the tooth root reached normal physiological length, and PCNA was positively expressed in the HERS cells of the terminal part. Similar to the normal mouse, the gene knockout mouse also formed a HERS structure on postnatal day 5. However, HERS began to break on postnatal day 9. On postnatal day 19, only a few fragments of HERS were found on the root surface, and the root development was immature. Moreover, the expression intensity of PCNA in the gene knockout mouse was decreased.

Conclusion

The Vps4b gene mutation may change the CK14 and PCNA expressions, leading to abnormal root development.

Key words: Vps4b gene, Hertwig's epithelial root sheath, cytokeratin 14, proliferating cell nuclear antigen, gene knockout

中图分类号:

Q 78

田晴, 王莹莹, 李强, 陈栋. Vps4b基因敲除鼠上皮根鞘中细胞角蛋白14和增殖细胞核抗原的表达[J]. 华西口腔医学杂志, 2021, 39(3): 274-278.

Tian Qing, Wang Yingying, Li Qiang, Chen Dong. Expressions of cytokeratin 14 and proliferating cell nuclear antigen in the Hertwig’s epithelial root sheath of a Vps4b knockout mouse[J]. West China Journal of Stomatology, 2021, 39(3): 274-278.

图/表 4

参考文献 13

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基因型	引物序列（5'-3'）
Vps4b-1	F1：TGCTTTAGGCAAAGCAGCAC
	R1：GCTGAAACTGGAGGGTTAGG
Vps4b-2	F2：TGCTTTAGGCAAAGCAGCAC
	R2：TTTCTGTGTGAGGGAGGCAA

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Vps4b基因敲除鼠上皮根鞘中细胞角蛋白14和增殖细胞核抗原的表达

Expressions of cytokeratin 14 and proliferating cell nuclear antigen in the Hertwig’s epithelial root sheath of a Vps4b knockout mouse

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