华西口腔医学杂志 ›› 2012, Vol. 30 ›› Issue (4): 430-433.doi: 10.3969/j.issn.1000-1182.2012.04.025

• 专栏论著 • 上一篇    下一篇

周期性张应力作用下成骨样细胞MG-63中c-fos基因和丝状肌动蛋白相互关系的研究

堵安庆1,2 王羽3,4 赵森1,2 李炜鹏1,2 赵志河1,2     

  1. 1.四川大学华西口腔医院正畸科; 2.口腔疾病研究国家重点实验室, 四川大学, 成都610041;3.中山大学光华口腔医学院·附属口腔医院正畸科; 4.广东省口腔医学重点实验室, 广州510055
  • 收稿日期:2011-07-25 修回日期:2011-11-22 出版日期:2012-08-01 发布日期:2012-08-01
  • 通讯作者: 赵志河,Tel:028-85503645
  • 作者简介:堵安庆(1977—),男,广东人,主治医师,博士

The relationship between c-fos gene and filamentous actin cytoskeleton in MG-63 osteoblasts under cyclic tensile stress

Du Anqing1,2, Wang Yu3,4, Zhao Sen1,2, Li Weipeng1,2, Zhao Zhihe1,2.   

  1. 1. Dept. of Orthodontics, West China School of Stomatology, Sichuan University, Chengdu 610041, China; 2. State Key Laboratory of Oral Diseases,
    Sichuan University, Chengdu 610041, China; 3. Dept. of Orthodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangzhou 510055, China; 4. Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China
  • Received:2011-07-25 Revised:2011-11-22 Online:2012-08-01 Published:2012-08-01

摘要:

目的探讨成骨样细胞MG-63中c-fos基因和细胞骨架丝状肌动蛋白(F-actin)的相互关系。方法对MG-63细胞施加周期性张应力,频率为0.5 Hz,细胞应变为2 000 μstrain,按3、6、12 h不同时间段进行加载,采用荧光定量聚合酶链反应(PCR)检测c-fos mRNA的变化,筛选最佳加载时间点,并作为实验组和0 h组进行对比,检测在细胞松弛素D作用下c-fos mRNA和F-actin的表达变化。结果周期性张应力可以诱导c-fos mRNA的表达增加,至3 h达到峰值;在周期性张应力作用下,MG-63细胞中F-actin的结构、排列发生改变,但荧光强度无明显变化;经细胞松弛素D处理后,张应力作用下的MG-63细胞的应力纤维明显减少,F-actin荧光强度降低,c-fos mRNA表达受抑制。结论周期性张应力作用下,F-actin的量并没有明显变化,只是出现了结构上的重组,且重组的是既有的F-actin。F-actin重组是应力诱导c-fos基因高表达的一个重要环节。

关键词: 周期性张应力, 成骨细胞, c-fos基因, 丝状肌动蛋白

Abstract:

Objective To investigate the relationship between c-fos gene and filamentous actin(F-actin) in MG-63 osteoblasts under cyclic tensile stress. Methods MG-63 osteoblasts were subjected to cyclic tensile strss(0.5 Hz, 2 000 μstrain) for 3, 6, and 12 h. The changes of c-fos gene were investigated by fluorescent quantitation polymerase chain reaction. Then the best loading time group was screened as the experimental group compared with 0 h group. The changes of F-actin and c-fos were investigated with or without cytochalasin D treatment. Results Cyclic tensile stress induced high expression of c-fos mRNA, and peaked at 3 h. After loading, F-actin had a structure reorganization, but had no change in expression. After cytochalasin D treatment, the formation of stress fibers and the fluorescence intensity of F-actin cytoskeleton significantly reduced, meanwhile the c-fos mRNA expression was inhibited. Conclusion After loading, there is only structure reorganization for F-actin, and the expression has not any change. That means the remodeling F-actin is the existing one. F-actin reorganization is an important part in c-fos gene expression induced by stress.

Key words: cyclic tensile stress, osteoblasts, c-fos gene, filamentous actin