Alteration of metabolic characteristics on the masseter muscle fiber of unilateral chewing rats and its adenosine mono-phosphate activated protein kinase regulatory mechanism

doi:10.7518/hxkq.2017.03.006

Abstract

Abstract:

Objective This study aims to determine the influence of unilateral chewing on metabolic characteristics of masseter muscle fibers in rats and the regulatory effect of an adenosine monophosphate activated protein kinase (AMPK) signal pathway on metabolism. Methods Rats were submitted to exodontia of all the right maxillary molars and divided into 2, 4, 6, and 8 weeks groups, and corresponding control groups were set as well. Sections were stained by nicotine adenine dinucleotide tetrazolim reductase(NADH-TRase) to demonstrate the types, proportion, and density of masseter muscle fibers. AMPKα1 and p-AMPK(Thr172) levels in bilateral masseter muscles were detected by Western blot. Results In the 2-week group, the percentage of dark fibers augmented in the ipsilateral side, whereas the percentage of intermediary fibers in the contralateral side was increased accompanied by a decrease of light fibers, compared with the control group (P<0.05). The percentage of dark fibers was increased in the bilateral sides, whereas the percentage of dark fiber in the ipsilateral sides surpassed that of the contralateral sides in the 4, 6, and 8-week groups. The percentage of intermediary fibers was decreased in the bilateral sides in the 6 and 8-week groups (P<0.05). The percentage of light fibers was reduced in the ipsilateral sides in the 8-week group, whereas no alteration was observed in contralateral sides (P>0.05). In the ipsilateral sides, p-AMPK (Thr172)/AMPKα1 levels were increased in the 2 and 4-week groups (P<0.05), whereas no change was observed in the con-tralateral sides in either group (P>0.05). Conclusion Uni-lateral chewing increases the oxidative metabolic ability in bilateral masseter muscle fibers especially in the non-working side accompanied with change of muscle fiber types. The improvement of aerobic metabolism ability is related to the AMPK signal pathway.

Key words: nicotine adenine dinucleotide tetrazolium reductase, adenosine monophosphate activated protein kinase, oxidative metabolism, unilateral chewing

CLC Number:

Q 257

Andi Shi, Lin Zeng, Jing Liu. Alteration of metabolic characteristics on the masseter muscle fiber of unilateral chewing rats and its adenosine mono-phosphate activated protein kinase regulatory mechanism[J]. West China Journal of Stomatology, 2017, 35(3): 258-263.

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时间	组别	深染	中染	浅染
2周	实验组拔牙侧	26.07±1.02^acde	43.02±2.71^de	30.59±2.59^e
	实验组非拔牙侧	22.31±1.93	54.30±1.47^acd	22.40±1.57^acd
	对照组右侧	20.59±2.10	45.87±2.87	33.53±1.16
	对照组左侧	21.65±1.15	45.03±2.99	33.43±1.83
4周	实验组拔牙侧	31.33±2.26^abde	41.79±2.84^ade	27.52±5.14
	实验组非拔牙侧	26.08±1.69^a	48.31±0.97^bd	25.63±2.85^abd
	对照组右侧	20.98±1.77	45.43±1.60	33.58±1.31
	对照组左侧	19.89±2.17	46.69±1.56	33.40±2.40
6周	实验组拔牙侧	39.90±1.94^abce	30.86±1.41^ae	29.84±5.26
	实验组非拔牙侧	26.59±4.36	40.32±1.58^abc	32.97±2.17^bc
	对照组右侧	21.57±1.87	44.82±3.02	33.63±1.78
	对照组左侧	20.37±2.02	46.25±2.98	33.37±1.36
8周	实验组拔牙侧	35.20±2.82^abcde	39.97±4.98^ad	24.33±1.57^abe
	实验组非拔牙侧	26.08±1.69^a	40.92±2.87^abc	33.27±1.10^bc
	对照组右侧	20.45±2.64	46.81±1.23	32.73±1.70
	对照组左侧	19.68±1.17	47.91±2.15	32.40±2.33

组别	2周	4周	6周	8周
对照组	1	0.99±0.07	1.05±0.11	1.14±0.04
实验组非拔牙侧	0.77±0.33	1.19±0.26	1.22±0.36	1.28±0.55
实验组拔牙侧	1.50±0.17^ade	1.31±0.30^a	1.12±0.29^b	0.87±0.11^bcd