Abstract:

Objective To study the effect of stable transfection of small hairpin RNA（shRNA） targeting Survivin gene on the growth of adenoid cystic carcinoma（ACC） xenografts in nude mice. Methods Adenoid cystic carcinoma cell-2 line（ACC-2） was transfected with Survivin targeting shRNA eukaryotic expression vector, and stable monoclonal cells were selected. Transfected and nontransfected cells were respectively inoculated into subcutaneous tissue of nude mice as experimental and controls. Survivin mRNA expression of transfected and nontransfected cells were detected by reverse transcription -polymerse chain reaction（RT -PCR）. Survivin protein expression of tumors was investigated by immunohistochemistry. The growth of xenograft tumors was observed by measurement of tumor volume. Results Compared to controls, the expression of tumor volume decreased in transfected group by pGenesil-shRNA-Survivin. The expression of Survivin mRNA and protein was obviously reduced. Conclusion Survivin siRNA markedly suppressed the growth of adenoid cystic carcinoma cell xenografts in nude mice through lastingly inhibition effect on Survivin.

Key words: RNA interference, short hairpin RNA, adenoid cystic carcinoma