Abstract:

Objective To observe the production of interleukin（IL）-1β, IL-6 and tumor necrosis factor α（TNFα） from stimulated human fibroblast with abstract from cell wall of Actinomyces naeslundii ATCC19246. Methods The abstract from the cell wall from Actinomyces naeslundii were extracted and purified with the method of purifying lipoteichoic acid（LTA） and stimulated the THP-1 with three different concentrations（1, 10, 100 mg/mL）. The level of IL-1β, IL-6 and TNFα in the supernatant was quantitatively analyzed by ELISA. Results Abstracts at the concentrations of 10, 100 mg/mL significantly produced IL-1β, IL-6 and TNFα, especially 10 mg/mL. Conclusion The abstract from cell wall of Actinomyces naeslundii may significantly increase IL-1β, IL-6 and TNFα level in the supernatant of THP-1, and the increasing level is different with the concentrations.

Key words: Actinomycesnaeslundii, interleukin, tumornecrosisfactorα, fibroblast