Abstract:

Objective To study the biological properties of human dental pulp cells（HDPC）by cloning and analysis of genes differentially expressed in HDPC in comparison with human gingival fibroblasts（HGF）. Methods HDPC and HGF were cultured and identified by immunocytochemistry. HPDC and HGF subtractive cDNA library was estab - lished by PCR- based modified subtractive hybridization, genes differentially expressed by HPDC were cloned, se - quenced and compared to find homogeneous sequence in GenBank by BLAST. Results Cloning and sequencing analysis indicate 12 genes differentially expressed were obtained, in which two were unknown genes. Among the 10 known genes, 4 were related to signal transduction, 2 were related to trans-membrane transportation（both cell membrane and nuclear membrane）, and 2 were related to RNA splicing mechanisms. Conclusion The biological properties of HPDC are determined by the differential expression of some genes and the growth and differentiation of HPDC are associated to the dynamic protein synthesis and secretion activities of the cell.

Key words: dental pulp cell, gingival fibroblast, differential expression, subtractive hybridization