Influence of Vascular Cell Co-cultured with Osteoblastin vitroon Ability of Osteoblast

West China Journal of Stomatology

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Influence of Vascular Cell Co-cultured with Osteoblastin vitroon Ability of Osteoblast

ZHANGJian1, HUMin1, ZHANG Wen-yi2

1.Dept. of Oral and Maxillofacial Surgery, PLA General Hospital, Beijing100853,China;2. Dept. of Oral and Maxillofacial Surgery,Hospital ofStomatology, Affiliated to Jinzhou Medical College, Jinzhou121000,Chin

Received:2005-08-25 Revised:2005-08-25 Online:2005-08-20 Published:2005-08-20

Abstract

Abstract: Objective　The purpose of this study was to observe the influence on proliferation of rabbit osteoblast (ROB) of rabbit vascular endothelial cell(RVEC) co-culturedwith rabbit osteoblast (ROB).Methods　Rabbit osteoblasts and rabbit vascu- lar endothelial cells in ratio of 1∶0,1∶1,2∶1,4∶1were selected to co-culture.The function of osteoblasts was assessed by alkaline phosphatase(ALP) activity and osteocalcin(OC) assay.Results　There was good compatibilitywhen osteoblasts and vascular en- dothelial cells were co-cultured. The activity ofALP and OC in the group of ROB and RVEC co-cultured in ratio of 2∶1 was higher than that of the other three groups.Conclusion　In direct co-culture systemin vitro, RVEC can improve activity of ROB.

Key words: osteoblast, vascular endothelial cell, alkaline phosphatase, osteocaclin

ZHANGJian1, HUMin1, ZHANG Wen-yi2. Influence of Vascular Cell Co-cultured with Osteoblastin vitroon Ability of Osteoblast[J]. West China Journal of Stomatology.

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Influence of Vascular Cell Co-cultured with Osteoblastin vitroon Ability of Osteoblast

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